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Injection
Injections are a fundamental step in chromatography and play a decisive role in the accuracy and reproducibility of analyses. During injection, a defined amount of the sample is introduced into the chromatographic system, where it is then separated into its components and analysed.
There are various methods of injection, including manual and automatic injections. Manual injections require precise manual labour to introduce the sample into the system using a microsyringe. Automatic injectors, on the other hand, improve efficiency and accuracy by introducing the sample consistently and reproducibly, which is particularly beneficial in high-throughput laboratories.
Precise injection is essential to ensure uniform and complete separation of sample components. Errors during injection can lead to inaccurate results and poor reproducibility. Therefore, well-calibrated injection systems and the use of high-quality injection needles and syringes are crucial.
Overall, injection is a critical step in the chromatographic process that significantly influences the quality and reliability of the analytical data.
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