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Tosoh Bioscience / TSKgel

TSKgel

TSKgel columns, manufactured by Tosoh Bioscience incorporate an extensive range of columns with either silica or polymer packings. Both high and low pressure TSKgel columns are available covering all modes of liquid chromatography, including reversed-phase, ion exchange, ion chromatography, size exclusion, affinity, hydrophobic interaction (HIC) and hydrophilic interaction (HILIC). Column dimensions from analytical to preparative scale are available.

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Chemistry Producers Term
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Items 91-100 of 596

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TSKgel ODS-100Z, 5 µm, 100 Å 150 mm x 3.0 mm
TSH-22759
TSKgel ODS-100Z, 5 µm, 100 Å 100 mm x 3.0 mm
TSH-22758
TSKgel ODS-100Z, 5 µm, 100 Å 75 mm x 3.0 mm
TSH-22757
TSKgel ODS-100Z, 5 µm, 100 Å 50 mm x 3.0 mm
TSH-22756
TSKgel ODS-100Z, 5 µm, 100 Å 35 mm x 3.0 mm
TSH-22755
TSKgel ODS-100Z, 5 µm, 100 Å 20 mm x 3.0 mm
TSH-22754
TSKgel ODS-100Z, 5 µm, 100 Å 250 mm x 2.0 mm
TSH-22753
TSKgel ODS-100Z, 5 µm, 100 Å 100 mm x 2.0 mm
TSH-22752
TSKgel ODS-100Z, 5 µm, 100 Å 75 mm x 2.0 mm
TSH-22751
TSKgel ODS-100Z, 5 µm, 100 Å 35 mm x 2.0 mm
TSH-22750
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Items 91-100 of 596

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Technical Data

Reversed-Phase Chromatography (RPC)

TSKgel RPC columns are based on high-purity, spherical silica gel. TSKgel ODS-100V is a proprietary monomeric bonded phase, provides complete wetting and retention stability in 100% aqueous mobile phases.

Phase-Name

Modification

Particle Size

Pore Size

End-capping

C-Content

USP

Surface

pH-Range

Tmax

TSKgel ODS-100V

C18

3, 5 µm

100 Å

+

15%

L1

450 m2/g

2.0‒7.5

N/A

TSKgel ODS-100Z

C18

3, 5 µm

100 Å

+

20%

L1

450 m2/g

2.0‒7.5

N/A

TSKgel OligoDNA RP

C18

5 µm

250 Å

-

10%

L1

N/A

2.0‒7.5

N/A

TSK ODS-140HTP

C18

2.3 µm

140 Å

+

8%

L1

N/A

2.0‒7.5

N/A

TSKgel Super-ODS

C18

2.3 µm

110 Å

+

8%

L1

N/A

2.0‒7.5

N/A

TSKgel ODS 80TS

C18

5, 10 µm

80 Å

+

15%

L1

N/A

2.0‒7.5

N/A

TSKgel ODS 80TM

C18

5, 10 µm

80 Å

+

15%

L1

N/A

2.0‒7.5

N/A

TSKgel ODS-120A

C18

5, 10 µm

120 Å

-

20%

L1

N/A

2.0‒7.5

N/A

TSKgel ODS-120T

C18

5, 10 µm

120 Å

+

22%

L1

N/A

2.0‒7.5

N/A

TSKgel Super-Octyl

C8

2.3 µm

110 Å

+

5%

L7

N/A

2.0‒7.5

N/A

TSKgel Octyl 80TS

C8

5 µm

80 Å

+

11%

L7

N/A

2.0‒7.5

N/A

TSKgel Protein C4-300

C4

3 µm

300 Å

+

3%

L26

100 m2/g

2.0‒7.5

N/A

TSKgel TMS 250

C1

10 µm

250 Å

+

5%

L13

N/A

1.5‒7.5

N/A

TSKgel Super-Phenyl

Phenyl

2.3 µm

110 Å

+

3%

L11

N/A

2.0‒7.5

N/A

TSKgel CN 80TS

Cyano

5 µm

80 Å

+

8%

L10

N/A

2.0‒7.5

N/A

Polymer-based TSKgel RPC columns are based on polymethacrylate and are chemically stable at pH=2-12, allowing operation at basic pH where silica-based columns have limited stability. Polymer-based columns can be cleaned and impurities removed by using either strong acid or base.

Phase-Name

Modification

Particle Size

Pore Size

End-capping

C-Content

USP

Surface

pH-Range

Tmax

TSKgel Octadecyl-NPR

C18

2.5 µm

Non-porous

-

N/A

N/A

N/A

2.0‒12.0

N/A

TSKgel Octadecyl-2PW

C18

5 µm

125 Å

-

N/A

N/A

N/A

2.0‒12.0

N/A

TSKgel Octadecyl-4PW

C18

7, 13 µm

500 Å

-

N/A

N/A

N/A

2.0‒12.0

N/A

TSKgel Phenyl-5PW

Phenyl

10, 13 µm

1000 Å

-

N/A

N/A

N/A

2.0‒12.0

N/A

Size Exclusion Chromatography (SEC)

TSKgel SW-Type columns are based on silica gel and are well-suited for the analysis of proteins and nucleic acids using aqueous buffer as mobile phase. The TSKgel SW mAb columns within the TSKgel SW series are specifically designed for the analysis of monoclonal antibodies.

Phase-Name

Modification

Particle Size

Pore Size

pH-Range

Tmax

Exclusion Limit (Globular Proteins)

Exclusion Limit (Dextrans)

TSKgel G2000SW

Diol

10, 13 µm

125 Å

2.5‒7.5

30 °C

5.000 ‒ 150.000 Da

1.000 ‒ 30.000 Da

TSKgel G3000SW

Diol

10, 13 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

2.000 ‒ 70.000 Da

TSKgel G4000SW

Diol

13, 17 µm

450 Å

2.5‒7.5

30 °C

20.000 ‒ 7.000.000 Da

4.000 ‒ 500.000Da

TSKgel G2000SWXL

Diol

5 µm

125 Å

2.5‒7.5

30 °C

5.000 ‒ 150.000 Da

1.000 ‒ 30.000 Da

TSKgel G3000SWXL

Diol

5 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

2.000 ‒ 70.000 Da

TSKgel G4000SWXL

Diol

8 µm

450 Å

2.5‒7.5

30 °C

20.000 ‒ 7.000.000 Da

4.000 ‒ 500.000Da

TSKgel BioAssist G2SWXL*

Diol

5 µm

125 Å

2.5‒7.5

30 °C

5.000 ‒ 150.000 Da

1.000 ‒ 30.000 Da

TSKgel BioAssist G3SWXL*

Diol

5 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

2.000 ‒ 70.000 Da

TSKgel BioAssist G4SWXL*

Diol

8 µm

450 Å

2.5‒7.5

30 °C

20.000 ‒ 7.000.000 Da

4.000 ‒ 500.000Da

TSKgel QC-PAK GFC 200

Diol

5 µm

125 Å

2.5‒7.5

30 °C

5.000 ‒ 150.000 Da

1.000 ‒ 30.000 Da

TSKgel QC-PAK GFC 300

Diol

5 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

2.000 ‒ 70.000 Da

TSKgel SuperSW2000

Diol

4 µm

125 Å

2.5‒7.5

30 °C

5.000 ‒ 150.000 Da

1.000 ‒ 30.000 Da

TSKgel SuperSW3000

Diol

4 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

2.000 ‒ 70.000 Da

TSKgel SuperSW mAb HR

Diol

4 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

N/A

TSKgel SuperSW mAb HTP

Diol

4 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

N/A

TSKgel UltraSW Aggregate

Diol

3 µm

300 Å

2.5‒7.5

30 °C

10.000 ‒ 2.000.000 Da

N/A

TSKgel UP-SW3000

Diol

2 µm

250 Å

2.5‒7.5

30 °C

10.000 ‒ 500.000 Da

N/A

* PEEK-Column-Hardware

TSKgel PW-Type columns are based on hydrophilic, rigid, spherical, porous methacrylate beads. They are commonly used for the separation of synthetic polymers, oligosaccharides, nucleic acids and small viruses using aqueous buffer or salt solutions as mobile phase, with up to 50% organic solvent.

Phase-Name

Modification

Particle Size

Pore Size

pH-range

Tmax

Exclusion Limit (Globular Proteins)

Exclusion Limit (Dextrans)

TSKgel G2000PW

-

12 µm

125 Å

2.0‒12.0

80 °C

< 2.000 Da

N/A

TSKgel G2500PW

-

12, 17 µm

< 200 Å

2.0‒12.0

80 °C

< 3.000 Da

< 3.000 Da

TSKgel G3000PW

-

12, 17 µm

200 Å

2.0‒12.0

80 °C

< 50.000 Da

< 60.000 Da

TSKgel G4000PW

-

17 µm

500 Å

2.0‒12.0

80 °C

< 300.000 Da

1.000 ‒ 700.000 Da

TSKgel G5000PW

-

17 µm

1000 Å

2.0‒12.0

80 °C

< 1.000.000 Da

50.000 ‒ 2.500.000 Da

TSKgel G6000PW

-

17 µm

> 1000 Å

2.0‒12.0

80 °C

< 8.000.000 Da

500.000 ‒ 50.000.000 Da

TSKgel GMPW

-

17 µm

< 100‒1000 Å

2.0‒12.0

80 °C

500 ‒ 8.000.000 Da

< 50.000.000 Da

TSKgel BioAssist G6PW

-

17 µm

> 1000 Å

2.0‒12.0

80 °C

< 8.000.000 Da

500.000 ‒ 50.000.000 Da

TSKgel G2500PWXL

-

7 µm

< 200 Å

2.0‒12.0

80 °C

N/A

< 3.000 Da

TSKgel G3000PWXL

-

7 µm

200 Å

2.0‒12.0

80 °C

< 50.000 Da

< 60.000 Da

TSKgel G4000PWXL

-

10 µm

< 500 Å

2.0‒12.0

80 °C

< 300.000 Da

1.000 ‒ 700.000 Da

TSKgel G5000PWXL

-

10 µm

1000 Å

2.0‒12.0

80 °C

< 1.000.000 Da

50.000 ‒ 2.500.000 Da

TSKgel G6000PWXL

-

13 µm

> 1000 Å

2.0‒12.0

80 °C

< 8.000.000 Da

500.000 ‒ 50.000.000 Da

TSKgel G-DANN-PW

-

10 µm

> 1000 Å

2.0‒12.0

50 °C

< 8.000.000 Da

< 50.000.000 Da

TSKgel GMPWXL

-

13 µm

100‒1000 Å

2.0‒12.0

80 °C

500 ‒ 8.000.000 Da

< 50.000.000 Da

TSKgel G-Oligo-PW

-

7 µm

125 Å

2.0‒12.0

80 °C

0 < 3.000 Da

N/A

TSKgel SuperMultipore PW-N

-

4 µm

N/A

2.0‒12.0

80 °C

300 ‒ 50.000 Da

N/A

TSKgel SuperMultipore PW-M

-

5 µm

N/A

2.0‒12.0

80 °C

500 ‒ 1.000.000 Da

N/A

TSKgel SuperMultipore PW-H

-

8 µm

N/A

2.0‒12.0

80 °C

1.000 ‒ 10.000.000 Da

N/A

TSKgel SuperOligoPW

-

3 µm

125 Å

2.0‒12.0

80 °C

100 ‒ 3.000 Da

N/A

TSKgel G3000PWXL-CP

-

7 µm

200 Å

2.0‒12.0

80 °C

< 90.000 Da

N/A

TSKgel G5000PWXL-CP

 

10 µm

1000 Å

2.0‒12.0

80 °C

< 1.000.000 Da

N/A

TSKgel G6000PWXL-CP

 

13 µm

> 1000 Å

2.0‒12.0

80 °C

< 20.000.000 Da

N/A

TSKgel Alpha and SuperAW columns are based on highly crosslinked polymethacrylate. The columns are compatible with a wide range of solvents (from 100% water to 100% non-polar organic solvents) and were developed for the GPC analysis of polymers of intermediate polarity, soluble in water, buffers and many organic solvents. 

Phase-Name

Modification

Particle size

Pore Size

pH-Range

Tmax

Exclusion Limit (PEO)

Exclusion Limit (Dextrans)

TSKgel Alpha-2500

-

7 µm

25 Å

2.0‒12.0

80 °C

5.000 Da

10.000 Da

TSKgel Alpha-3000

-

7 µm

150 Å

2.0‒12.0

80 °C

90.000 Da

100.000 Da

TSKgel Alpha-4000

-

10 µm

450 Å

2.0‒12.0

80 °C

400.000 Da

1.000.000 Da

TSKgel Alpha-5000

-

10 µm

1000 Å

2.0‒12.0

80 °C

1.000.000 Da

7.000.000 Da

TSKgel Alpha-6000

-

13 µm

N/A

2.0‒12.0

80 °C

> 10.000.000 Da

> 10.000.000 Da

TSKgel Alpha-M

-

13 µm

N/A

2.0‒12.0

80 °C

> 10.000.000 Da

> 10.000.000 Da

TSKgel Super AW 2500

-

4 µm

25 Å

2.0‒12.0

80 °C

5.000 Da

8.000 Da

TSKgel Super AW 3000

-

4 µm

150 Å

2.0‒12.0

80 °C

90.000 Da

80.000 Da

TSKgel Super AW 4000

-

6 µm

450 Å

2.0‒12.0

80 °C

1.000.000 Da

600.000 Da

TSKgel Super AW 5000

-

7 µm

1000 Å

2.0‒12.0

80 °C

1.000.000 Da*

N/A

TSKgel Super AW 6000

-

9 µm

N/A

2.0‒12.0

80 °C

10.000.000 Da*

N/A

TSKgel Super AW M-H

-

9 µm

N/A

2.0‒12.0

80 °C

10.000.000 Da*

N/A

* Estimated Values

TSKgel H-Type columns are recommended for the analysis of organic soluble polymers and are packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB). The packings are available in eight pore sizes and span four different columns chemistries. For polymer samples with a broad molecular range, packing of several pore sizes are provided in the mixed bed columns: TSKgel SuperHZM series, TSKgel SuperHM series, TSKgel GMHXL, TSKgel GMHHR, and selected high temperature versions provide linear calibration curves up to several million Daltons.

Phase-Name

Modification

Particle Size

Pore Size

pH-Range

Tmax

Exclusion Limit (Polystyrene)

TSKgel G1000HHR

-

5 µm

15 Å

1.0‒14.0

140 °C

< 1.000 Da

TSKgel G2000HHR

-

5 µm

20 Å

1.0‒14.0

140 °C

< 10.000 Da

TSKgel G2500HHR

-

5 µm

30 Å

1.0‒14.0

140 °C

20.000 Da

TSKgel G3000HHR

-

5 µm

75 Å

1.0‒14.0

140 °C

< 60.000 Da

TSKgel G4000HHR

-

5 µm

200 Å

1.0‒14.0

140 °C

< 400.000 Da

TSKgel G5000HHR

-

5 µm

650 Å

1.0‒14.0

140 °C

< 4.000.000 Da

TSKgel G6000HHR

-

5 µm

N/A

1.0‒14.0

140 °C

< 40.000.000 Da

TSKgel G7000HHR

-

5 µm

N/A

1.0‒14.0

140 °C

400.000.000 Da

TSKgel GMHHR-L mixed-bed

-

5 µm

N/A

1.0‒14.0

140 °C

< 4.000.000 Da

TSKgel GMHHR-N mixed-bed

-

5 µm

N/A

1.0‒14.0

140 °C

< 4.000.000 Da

TSKgel GMHHR-M mixed-bed

-

5 µm

N/A

1.0‒14.0

140 °C

< 4.000.000 Da

TSKgel GMHHR-H mixed-bed

-

5 µm

N/A

1.0‒14.0

140 °C

< 400.000.000 Da

TSKgel G1000HXL

-

5 µm

15 Å

1.0‒14.0

60 °C

1.000 Da

TSKgel G2000HXL

-

5 µm

20 Å

1.0‒14.0

60 °C

10.000 Da

TSKgel G2500HXL

-

5 µm

30 Å

1.0‒14.0

60 °C

20.000 Da

TSKgel G3000HXL

-

5 µm

75 Å

1.0‒14.0

60 °C

60.000 Da

TSKgel G4000HXL

-

5 µm

200 Å

1.0‒14.0

60 °C

400.000 Da

TSKgel G5000HXL

-

9 µm

650 Å

1.0‒14.0

80 °C

4.000.000 Da

TSKgel G6000HXL

-

9 µm

N/A

1.0‒14.0

80 °C

40.000.000 Da

TSKgel G7000HXL

-

9 µm

N/A

1.0‒14.0

80 °C

400.000.000 Da

TSKgel GMHXL mixed-bed

-

9 µm

N/A

1.0‒14.0

80 °C

400.000.000 Da

TSKgel GMHXL-L mixed-bed

-

5 µm

N/A

1.0‒14.0

80 °C

4.000.000 Da

TSKgel Multipore HXL-M

-

5 µm

N/A

1.0‒14.0

80 °C

2.000.000 Da

TSKgel SuperH1000

-

3 µm

15 Å

1.0‒14.0

140 °C

1.000 Da

TSKgel SuperH2000

-

3 µm

20 Å

1.0‒14.0

140 °C

10.000 Da

TSKgel SuperH2500

-

3 µm

30 Å

1.0‒14.0

140 °C

20.000 Da

TSKgel SuperH3000

-

3 µm

75 Å

1.0‒14.0

140 °C

60.000 Da

TSKgel SuperH4000

-

3 µm

200 Å

1.0‒14.0

140 °C

400.000 Da

TSKgel SuperH5000

-

3 µm

650 Å

1.0‒14.0

140 °C

4.000.000 Da

TSKgel SuperH6000

-

5 µm

N/A

1.0‒14.0

140 °C

40.000.000 Da

TSKgel SuperH7000

-

5 µm

N/A

1.0‒14.0

140 °C

400.000.000 Da

TSKgel SuperHM-L

-

3 µm

N/A

1.0‒14.0

140 °C

4.000.000 Da

TSKgel SuperHM-N

-

3 µm

N/A

1.0‒14.0

140 °C

400.000 Da

TSKgel SuperHM-M

-

3 µm

15 Å

1.0‒14.0

140 °C

2.000.000 Da

TSKgel SuperHM-H

-

3 µm

20 Å

1.0‒14.0

140 °C

400.000.000 Da

TSKgel SuperHZ1000

-

3 µm

30 Å

1.0‒14.0

60 °C

1.000 Da

TSKgel SuperHZ2000

-

3 µm

75 Å

1.0‒14.0

60 °C

10.000 Da

TSKgel SuperHZ2500

-

3 µm

200 Å

1.0‒14.0

60 °C

20.000 Da

TSKgel SuperHZ3000

-

3 µm

N/A

1.0‒14.0

60 °C

60.000 Da

TSKgel SuperHZ4000

-

3 µm

N/A

1.0‒14.0

80 °C

400.000 Da

TSKgel SuperHZM-N

-

3 µm

N/A

1.0‒14.0

80 °C

7.000.000 Da

TSKgel SuperHZM-M

-

3, 5 µm

140 Å

1.0‒14.0

80 °C

400.000.000 Da

TSKgel SuperHZM-H

-

10 µm

80 Å

1.0‒14.0

80 °C

400.000.000 Da

TSKgel SuperMultiporeHZ-M

-

4 µm

N/A

1.0‒14.0

N/A

2.000.000 Da

TSKgel SuperMultiporeHZ-N

-

3 µm

N/A

1.0‒14.0

N/A

120.000 Da

TSKgel SuperMultiporeHZ-H

-

6 µm

N/A

1.0‒14.0

N/A

40.000.000 Da

TSKgel GMHHR-H (30) HT2

-

30 µm

N/A

1.0‒14.0

220 °C

400.000.000 Da

TSKgel GMHHR-H (20) HT2

-

20 µm

N/A

1.0‒14.0

220 °C

400.000.000 Da

TSKgel GMHHR-H (S) HT2

-

13 µm

N/A

1.0‒14.0

220 °C

400.000.000 Da

TSKgel G2000HHR (20) HT2

-

20 µm

20 Å

1.0‒14.0

220 °C

10.000 Da

TSKgel GMHHR-H (30) HT

-

13 µm

N/A

1.0‒14.0

170 °C

400.000.000 Da

TSKgel GMHHR-H (20) HT

-

20 µm

N/A

1.0‒14.0

170 °C

400.000.000 Da

TSKgel GMHHR-H (S) HT

-

13 µm

N/A

1.0‒14.0

170 °C

400.000.000 Da

Ion Exchange Chromatography

There are different silica-based TSKgel SW-Type columns for Ion Exchange Chromatography with various modifications (SAX, WAX, SCX, WCX).

Phase-Name

Modification

Particle Size

Pore Size

pH-Range

Tmax

Exclusion Limit (PEG)

Capacity

TSKgel QAE-2SW

Trimethylamino

5 µm

125 Å

2.0‒7.5

N/A

10.000 Da

N/A

TSKgel DEAE-2SW

Diethylaminoethyl

5 µm

125 Å

2.0‒7.5

N/A

10.000 Da

N/A

TSKgel DEAE-3SW

Diethylaminoethyl

10 µm

250 Å

2.0‒7.5

N/A

30.000 Da

N/A

TSKgel SP-2SW

Sulfopropyl

5 µm

125 Å

2.0‒7.5

N/A

10.000 Da

N/A

TSKgel CM-2SW

Carboxymethyl

5 µm

125 Å

2.0‒7.5

N/A

10.000 Da

110 mg Hemoglobin/mL

TSKgel CM-3SW

Carboxymethyl

10 µm

250 Å

2.0‒7.5

N/A

30.000 Da

N/A

Polymethacrylate-based columns for SAX, WAX, SCX or WCX

Phase-Name

Modification

Particle Size

Pore Size

pH-Range

Tmax

Exclusion Limit (PEG)

Capacity

TSKgel BioAssist Q

Polyamine

10, 13 µm

4000 Å

2.0‒12.0

N/A

5.000.000 Da

70 mg BSA/mL

TSKgel BioAssist S

Sulfopropyl

7, 13 µm

1300 Å

2.0‒12.0

N/A

~ 4.000.000 Da

70 mg γ-Globulin

TSKgel SuperQ-5PW

Trimethylamino

10, 13 µm

1000 Å

2.0‒12.0

N/A

1.000.000 Da

100 mg BSA/mL

TSKgel DEAE-5PW

Diethylaminoethyl

10, 13, 20 µm

1000 Å

2.0‒12.0

N/A

1.000.000 Da

30 mg BSA/mL

TSKgel SP-5PW

Sulfopropyl

10, 13, 20 µm

1000 Å

2.0‒12.0

N/A

1.000.000 Da

40 mg Hemoglobin/mL

TSKgel CM-5PW

Carboxymethyl

10, 13 µm

1000 Å

2.0‒12.0

N/A

1.000.000 Da

45 mg Hemoglobin/mL

TSKgel Q-STAT

Trimethylamino

7, 10 µm

Non-porous

3.0‒10.0

N/A

500 Da

20 mg BSA/mL

TSKgel DNA-STAT

Trimethylamino

5 µm

Non-porous

3.0‒10.0

N/A

500 Da

35 mg BSA/mL

TSKgel SP-STAT

Sulfopropyl

7, 10 µm

Non-porous

3.0‒10.0

N/A

500 Da

10 mg Lysozyme/mL

TSKgel CM-STAT

Carboxymethyl

7, 10 µm

Non-porous

3.0‒10.0

N/A

500 Da

15 mg Lysozyme/mL

TSKgel DEAE-NPR

Diethylaminoethyl

2.5 µm

Non-porous

2.0‒12.0

N/A

500 Da

5 mg BSA/mL

TSKgel DNA-NPR

Proprietary

2.5 µm

Non-porous

2.0‒12.0

N/A

500 Da

5 mg BSA/mL

TSKgel SP-NPR

Sulfopropyl

2.5 µm

Non-porous

2.0‒12.0

N/A

500 Da

5 mg Hemoglobin/mL

PS-DVB based columns for Ion Exchange Chromatography

Phase-Name

Modification

Particle Size

Pore Size

pH-Range

Tmax

Exclusion Limit (PEG)

Capacity

TSKgel Sugar AXI

Trimethylamino

8 µm

60 Å

1.0‒14.0

N/A

N/A

N/A

TSKgel Sugar AXG

Trimethylamino

10 µm

60 Å

1.0‒14.0

N/A

N/A

N/A

TSKgel SAX

Trimethylamino

5 µm

60 Å

1.0‒14.0

N/A

N/A

N/A

TSKgel SCX

Sulfonic acid

5 µm

60 Å

1.0‒14.0

N/A

N/A

N/A

Hydrophilic Interaction Chromatography (HILIC)

TSKgel HILIC columns are well-suited for the analysis of polar and hydrophilic compounds. The unique polar phases can be operated over a broad range of mobile phase conditions. They are stable in 100% organic solvents and are able to separate many different types of polar molecules.  

Phase-Name

Modification

Particle Size

Pore Size

End-capping

C-Content

USP

Surface

pH-Range

Tmax

TSKgel Amide-80

Amide

2, 3, 5, 10 µm

100 Å

N/A

N/A

L68

450 m2/g

2.0‒7.5

80°C
50°C*

TSKgel NH2-100

Amino

3 µm

100 Å

N/A

N/A

L8

450 m2/g

2.0‒7.5

50 °C

* For 3 µm Particle Size

Hydrophobic Interaction Chromatography (HIC)

TSKgel HIC phases are polymethacrylate-based with a choice of three ligands with varied hydrophobicities from low to high. TSKgel Phenyl-5PW is the most hydrophobic and Butyl-NPR the least hydrophobic among the three TSKgel HIC columns. The phases are physically and chemically stable in water soluble organic solvents (at < 50% methanol, ethanol, ACN, DMF, DMSO or < 30% chloroform).

Phase-Name

Modification

Particle Size

Pore Size

End-capping

C-Content

USP

Surface

pH-Bereich

Tmax

TSKgel Phenyl-5PW

Phenyl

10 µm

1000 Å

N/A

N/A

N/A

N/A

2.0‒12.0

50 °C

TSKgel Etder-5PW

PEG

10 µm

1000 Å

N/A

N/A

N/A

N/A

2.0‒12.0

50 °C

TSKgel Butyl-NPR

Butyl

2.5 µm

Non-porous

N/A

N/A

N/A

N/A

2.0‒12.0

60 °C

Affinity Chromatography (AFC)

TSKgel Boronate-5PW carries m-aminophenyl boronate as ligand. Under alkaline conditions the boronate anionic structure of the TSKgel Boronate-5PW can bind with 1,2 cis-diol groups, such as those found in carbohydrates, carbohydrate-containing compounds and catecholamines. This interaction is enhanced in the presence of Mg2+ ions and inhibited by amine-containing buffers.
 

TSKgel Chelate utilizes the ability of iminodiacetic acid (IDA) to chelate ions such as Zn2+, Ni2+ and Cu2+. Peptides and Proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds are then eluted with buffer containing imidazole or glycine.
 

TSKgel Tresyl requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.
 

The TSKgel Protein A-5PW column has been designed for the rapid separation and robust quantification of a variety of antibodies.

Phase-Name

Modification

Particle Size

Pore Size

End-capping

C-Content

USP

Surface

pH-Bereich

Tmax

TSKgel Boronate-5PW

m-Aminophenyl-boronate

10 µm

1000 Å

N/A

N/A

N/A

N/A

2.0‒12.0

45 °C

TSKgel Chelate-5PW

Iminodiacetic acid

10 µm

1000 Å

N/A

N/A

N/A

N/A

2.0‒9.0

N/A

TSKgel Tresyl-5PW

Tresyl

10 µm

1000 Å

N/A

N/A

N/A

N/A

2.0‒12.0

N/A

TSKgel Protein A-5PW

Recombinant Protein A,
Hexamer of C Domain

20 µm

1000 Å

N/A

N/A

N/A

N/A

2.0‒12.0

30°C

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