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ProSwift
ProSwift™ are HPLC Columns for fast and high-resolution LC/MS separations in bottom-up protein identification, biomarker discovery, and systems biology applications. Columns for reversed-phase, ion-exchange and affinity chromatography are available.
ProSwift™ RP phases are on a monolithic polystyrene divinylbenzene copolymer. ProSwift™ C4 RP-5H and all other ProSwift™ phases are based on monolithic Polymethacrylate. Each monolith is a single cylindrical, sponge-like polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size; ranging from small channel (1S), medium size channels (2H % 4H) to very large channel (3U). These channels combined with the monolith's nonporous surfaces result in fast mass transfer for high resolution and fast protein separations. The channels also produce low backpressure, allowing the use of higher linear velocities with minimal loss of resolution.
The ProSwift™ RP-4H and PepSwift™ polymer matrices differ with respect to crosslink and co-monomer ratio, which may result in different selectivity under comparable conditions.
The ProSwift ConA-1S affinity monolith column is designed for the highly efficient enrichment and purification of Concanavalin A binding glycans, glycopeptides and glycoproteins.
Technical Data
Columns for reversed-phase chromatography:
| Phase name | Surface functionality | Particle size | Pore size | pH range | Tmax | Protein binding capacity |
| ProSwift™ RP-1S | phenyl | Monolithic | 1.1 µm | 1.0-14.0 | 70 °C | 3.0 mg lysozymes/mL |
| ProSwift™ RP-2H | phenyl | Monolithic | 2.2 µm | 1.0-14.0 | 70 °C | 1.0 mg lysozymes/mL |
| ProSwift™ RP-3U | Phenyl | Monolithic | 5.2 µm | 1.0-14.0 | 70 °C | 0.5 mg lysozymes/mL |
| ProSwift™ RP-4H | phenyl | Monolithic | N/A | 1.0-14.0 | 70 °C | 2.3 mg lysozymes/mL |
| ProSwift™ RP-10R | Phenyl | Monolithic | N/A | 1.0-10.0 | 80 °C | N/A |
| ProSwift™ C4 RP-5H | Butyl | Monolithic | N/A | 1.0-10.0 | 90 °C | N/A |
Columns for ion exchange chromatography:
| Phase name | Surface functionality | Particle size | Pore size | pH range | Tmax | Protein binding capacity |
| ProSwift™ WAX-1S | Tertiary amines | Monolithic | N/A | 2.0-12.0 | 60 °C | 18 mg BSA/mL |
| ProSwift™ WCX-1S | Carboxylic acid | Monolithic | N/A | 2.0-12.0 | 60 °C | 23 mg lysozymes/mL |
| ProSwift™ SAX-1S | Quaternary amines | Monolithic | N/A | 2.0-12.0 | 70 °C | 18 mg BSA/mL |
| ProSwift™ SCX-1S | Sulphonic acid | Monolithic | N/A | 2.0-12.0 | 60 °C | 13 mg lysozymes/mL |
Columns for affinity chromatography:
| Phase name | Surface functionality | Particle size | Pore size | Surface | pH range | Tmax |
| ProSwift™ ConA-1S | Concanavalin A | Monolithic | 0.62/3.25 µm | 5.5m2/g | 5.0-8.0 | 30 °C |
Downloads
Brochures
Thermo PepSwift ProSwift HPLC Brochure
Thermo ProSwift RP HPLC Life Science Brochure
Technical Support
Thermo ProSwift ConA-1S Product Manual HPLC Life-Science
Thermo ProsWift IEX Product Manual HPLC Life-Science
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