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Zenix columns are silica based columns for size exclusion chromatography (SEC). The silica particles are coated with a uniform, hydrophilic and neutral film. These columns can be used for separation of biological molecules and water soluble polymers in aqueous buffers.
Phase | Particle size | Pore size | Molecular range |
Zenix SEC-80 | 3 μm | 80 Å | 100 - 50,000 Da |
Zenix SEC-100 | 3 μm | 100 Å | 100 - 100,000 Da |
Zenix SEC-150 | 3 μm | 150 Å | 500 - 150,000 Da |
Zenix SEC-300 | 3 μm | 300 Å | 5,000 - 1,250,000 Da |
Recombinant Human Erythropoietin (CHO cell) rhEPO separation on Zenix SEC-150
1. Thyroglobulin, 670 kD
2. BSA dimer, 132 kD
3. BSA 66 kD
4. Ribonuclease A, 14 kD
5. Uracil, 120 Da
Test Conditions:
Column: ZenixTM SEC-150 (3 μm, 150 Å, 7.8 x 300 mm)
Mobile Phase: 150 mM phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV 280 nm
Column temperature: 25°C
Sample injection: 10 µL rhEPO 3 mg/mL in 100 mM NaCl, 0,3 mM Zitronensäure und 23 mM Natriumcitrat
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