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Polar
Polar columns from Sepax are columns for various purposes. The series includes both polymer and silica phases. Each phase has a special modification to fulfil different requirements.
Polar MC-HIC columns are made of hydrophilic polymethacrylate functionalized with ether or butyl for hydrophobic interaction chromatography (HIC).
Polar MC-IEX columns consist of hydrophilic methacrylate with ion exchange groups for ion exchange chromatography (IEX). The exchange groups are chemically bonded to the surface, creating a dense and uniform ion exchange layer.
Polar MC-IMAC columns are metal affinity chromatography columns for the purification of recombinant proteins with a histidine (His) tag and are based on hydrophilic polymethacrylate. The hydrophilic surface contains carboxylic acid groups that chelate polyvalent metal ions.
Polar-100 are silica-based columns with poly(ethylene glycol) as the functional group.
Polar imidazole columns consist of silica particles that have an imidazole functionalisation on their surface. This N-containing ring structure is a weak base and is therefore suitable for many applications, e.g. drugs, peptides, sugars and nucleotides.
Polar pyridine columns are silica-based columns in which a pyridine is chemically bound to the surface. This pyridine with its N-containing ring structure is a weak base and can be used for many different applications such as pharmaceuticals, peptides, sugars and nucleotides.
Technical Data
| Phase | Modification | Base material | Particle size | Pore size | Surface area | pH range | Endcapped |
| Polar MC30-HIC Butyl | C4 | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 2.0 - 14 | N/A |
| Polar MC30-HIC Ether | ether | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 2.0 - 14 | N/A |
| Polar MC30-SCX | N/A | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC60-SCX | N/A | Hydrophilic polymethacrylate | 45 - 90 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC30-WCX | N/A | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC60-WCX | N/A | Hydrophilic polymethacrylate | 45 - 90 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC30-SAX | N/A | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC60-SAX | N/A | Hydrophilic polymethacrylate | 45 - 90 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC30-WAX | N/A | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC60-WAX | N/A | Hydrophilic polymethacrylate | 45 - 90 µm | 800 Å | N/A | 2.0 - 12 | N/A |
| Polar MC30-CA Excel | Carboxylic acid | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 1.0 - 13 | N/A |
| Polar MC60-CA Excel | Carboxylic acid | Hydrophilic polymethacrylate | 45 - 90 µm | 800 Å | N/A | 1.0 - 13 | N/A |
| Polar MC30-Ni Excel | Carboxylic group/Ni2+ | Hydrophilic polymethacrylate | 20 - 45 µm | 800 Å | N/A | 1.0 - 13 | N/A |
| Polar MC60-Ni Excel | Carboxylic group/Ni2+ | Hydrophilic polymethacrylate | 45 - 90 µm | 800 Å | N/A | 1.0 - 13 | N/A |
| Polar-100 | Poly(ethylene glycol), polymeric | silica | 1.8, 3, 5, 10 µm | 120 Å | 300 m²/g | N/A | + |
| Polar diol | Diol, polymeric | silica | 1.8, 3, 5, 10 µm | 120 Å | 300 m²/g | N/A | + |
| Polar imidazoles | Imidazoles, polymeric | silica | 1.8, 3, 5, 10 µm | 120 Å | 300 m²/g | N/A | + |
| Polar pyridines | Pyridines, polymeric | silica | 1.8, 3, 5, 10 µm | 120 Å | 300 m²/g | N/A | + |
| Polar silica | silica | silica | 1.8, 3, 5, 10 µm | 60, 100, 120, 300, 500, 1000, 2000 Å | N/A | N/A | N/A |
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