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Carbomix
Carbomix columns consist of non-porous polystyrene divinylbenzene polymer. This polymer has sulfonic acid groups with various chelating metal ions on its surface. These columns are especially designed for high resolution separations of water soluble organic compounds such as carbohydrates, organic acids, peptides and small bio organic molecules.
Technical Data
| Phase | Counter ion | Particle size | Cross-linking | pH range | Tmax |
| Carbomix H-NP | H+ | 5, 10 μm | 5, 8, 10 % | 1.0-3.0 | 85 °C |
| Carbomix Ca-NP | Ca2+ | 5, 10 μm | 5, 8, 10 % | 5.0-9.0 | 85 °C |
| Carbomix Pb-NP | Pb2+ | 5, 10 μm | 5, 8, 10 % | 5.0-9.0 | 85 °C |
| Carbomix K-NP | K+ | 5, 10 μm | 5, 8, 10 % | 5.0-9.0 | 85 °C |
| Carbomix Na-NP | Na+ | 5, 10 μm | 5, 8, 10 % | 5.0-9.0 | 85 °C |
Applications
Retention time Table for Carbomix Phases
| Compound | Carbomix H-NP5 8% | Carbomix H-NP10 5% | Carbomix H-NP10 8% | Carbomix H-NP10 10% | Carbomix Ca-NP5 8% | Carbomix Ca-NP10 5% | Carbomix Ca-NP10 8% | Carbomix Ca-NP10% | Carbomix Pb-NP5 8% | Carbomix Pb-NP10 5% | Carbomix Pb-NP10 8% |
| Acetic acid | 14.11 | 17.94 | 15.33 | 13.70 | / | / | / | / | / | / | / |
| Adonitol | 10.39 | 13.73 | 11.76 | 9.97 | 14.706 | 16.7625 | 14.654 | 13.67 | 19.84 | 21.93 | 20.07 |
| Arabinitol | / | / | / | / | / | / | / | 16.06 | / | / | / |
| D-(-)-Arabinose | 10.36 | 13.76 | 11.71 | 9.89 | 13.6045 | 15.5905 | 13.3615 | 12.52 | 16.03 | 17.61 | 16.08 |
| L-(+)-Arabinose | 10.35 | 13.76 | 11.71 | 9.89 | 13.552 | 15.5945 | 13.379 | 12.53 | 16.02 | 17.58 | 16.12 |
| 1,4-Butanediol | 19.72 | 23.35 | 20.63 | 19.57 | 15.428 | 17.9025 | 15.412 | 14.48 | 16.67 | 17.66 | 16.64 |
| n-Butanol | 33.74 | 35.76 | 33.79 | 34.38 | 24.583 | 27.14 | 25.19 | / | 27.93 | 28.34 | 28.09 |
| t-Butanol | 23.21 | 26.46 | 23.8 | 23.43 | 16.2755 | 18.6255 | 16.243 | / | 16.97 | 17.71 | 16.95 |
| sec-butyl alcohol | 28.61 | 31.29 | 28.81 | 28.89 | 19.8715 | 22.497 | 20.095 | 19.01 | 21.26 | 22.35 | 21.37 |
| D-(+)-Cellobiose | 8.34 | 9.87 | 9.55 | 7.87 | 9.92 | 10.355 | 9.055 | 8.96 | 11.72 | 11.92 | 11.47 |
| Citric acid | 8.6 | 10.6 | 9.9 | 8.17 | / | / | / | / | / | / | / |
| Erythriyol | 11.27 | 14.77 | 12.62 | 10.86 | 15.368 | 17.3185 | 15.35 | 14.47 | 19.62 | 21.26 | 19.77 |
| Ethanol | 20.66 | 22.82 | 21.22 | 20.63 | 16.515 | 18.1795 | 16.394 | 15.89 | 17.08 |
| 17.09 |
| Formic acid | 13.08 | 16.46 | 14.3 | 12.67 | / | / | / | / | / | / | / |
| D-Fructose | 9.81 | 12.9 | 11.17 | 9.36 | 13.5535 | 15.4645 | 13.347 | 12.45 | 17.06 | 18.82 | 17.22 |
| Fumaric acid | 12.39 | 17.07 | 13.77 | 11.79 | / | / | / | / | / | / | / |
| Galactitol | 10.07 | 13.31 | 11.45 | 9.64 | 19.624 | 22.5455 | 20.343 | 18.05 | 31.46 | 35.88 | 32.43 |
| D-(+)-Galactose | 9.7 | 12.77 | 11.05 | 9.25 | 12.1835 | 13.963 | 11.809 | 11.2 | 14.85 | 16.35 | 14.88 |
| D-Glucose | 9.36 | 12 | 10.64 | 8.87 | 11.274 | 12.554 | 10.714 | 10.32 | 13.29 | 14.04 | 13.18 |
| Glycerol | 12.68 | 16.26 | 13.98 | 12.29 | 15.9965 | 17.8425 | 15.974 | 15.12 | 19.34 | 20.59 | 19.42 |
| IPA | 22.29 | 24.87 | 22.87 | 22.38 | 16.434 | 18.447 | 16.374 | 15.88 | 17.16 | 17.72 | 17.11 |
| Lactic acid | 11.93 | 15.69 | 13.27 | 11.51 | / | / | / | / | / | / | / |
| B-Lactose | 8.44 | 10.41 | 9.71 | 8.02 | 10.221 | 11.092 | 9.452 | 9.22 | 12.35 | 13.34 | 12.21 |
| D-Lyxose | 10.07 | 13.25 | 11.43 | 9.61 | 14.02775 | 16.167 | 13.922 | 13.02 | 16.5 | 18.04 | 16.61 |
| Maleic acid | 8.75 | 9.32 | 9.96 | 8.39 | / | / | / | / | / | / | / |
| Malic acid | 9.59 | 12.52 | 10.93 | 9.13 | / | / | / | / | / | / | / |
| Maltotetraose | / | / | / | / | / | / | / | / | / | / | / |
| Maltitol | 8.55 | 10.72 | 9.87 | 8.14 | 12.4125 | 15.0435 | 12.274 | 11.24 | 18.16 | 22.19 | 18.65 |
| D-(+)-Maltose | 8.37 | 10.15 | 9.65 | 7.94 | 10.064 | 10.665 | 9.268 | 9.09 | 12.19 | 12.8 | 12.01 |
| Maltotriose | / | / | / | / | / | / | / | 8.57 | / | / | / |
| D-Mannitol | 9.95 | 13.14 | 11.34 | 9.52 | 16.92 | 19.563 | 17.2445 | 15.6 | 24.23 | 27.79 | 24.86 |
| D-(+)-Mannose | 9.68 | 12.68 | 11.02 | 9.22 | 12.422 | 14.238 | 12.093 | 11.45 | 16.23 | 18.04 | 16.37 |
| D-(+)-Melezitose | 8.05 | 9.03 | 9.26 | 7.63 | 9.493 | 9.404 | 8.51 | 8.49 | 10.92 | 10.9 | 10.6 |
| Methanol | 18.45 | 20.61 | 19.14 | 18.36 | 16.325 | 17.7215 | 16.13 | 15.64 | 16.79 | 17.16 | 16.69 |
| Oxalic acid dihydrate | 7.95 | 7.53 | 9.12 | 7.63 | / | / | / | / | / | / | / |
| 1-Propanol | 25.24 | 27.48 | 25.63 | 25.57 | 19.332 | 21.2605 | 19.403 | 18.95 | 20.75 | 21.33 | 20.76 |
| 1,2-Propylene glycol | 15.89 | 19.45 | 17 | 15.6 | 17.307 | 18.8945 | 17.274 | / | 20.45 | 20.97 | 20.45 |
| Pyruvic acid | / | / | / | / | / | / | / | / | / | / | / |
| D-(-)-Ribose | 10.6 | 14.04 | 11.9 | 10.08 | 20.842 | 24.489 | 21.913 | 19.23 | 30.21 | 34.62 | 31.1 |
| D-Sorbito | 10.05 | 13.3 | 11.43 | 9.61 | 20.632 | 23.786 | 21.2095 | 18.71 | 33.51 | 39.07 | 34.72 |
| Succinic acid | 11.07 | 15.33 | 12.49 | 10.57 | / | / | / | / | / | / | / |
| D-(+)-Sucrose | / | / | / | / | 9.9875 | 10.594 | 9.175 | 9.03 | 11.74 | 12.12 | 11.51 |
| Tartartic | 8.93 | 11.06 | 10.23 | 8.49 | / | / | / | / | / | / | / |
| Triethylene glycol | / | / | / | / | / | / | / | 17.21 | / | / | / |
| Xylitol | 10.75 | 14.27 | 12.15 | 10.33 | 20.0725 | 23.067 | 20.79 | 18.66 | 30.7 | 35.15 | 31.54 |
| D-Xylose | 9.75 | 12.74 | 11.11 | 9.31 | 12.1335 | 13.6325 | 11.692 | 11.19 | 14.17 | 14.94 | 14.1 |
| Rhamnose | 10.09 | 13.41 | 11.46 | 9.64 | 12.4475 | 14.172 | 12.088 |
| 14.75 | 15.76 | 14.79 |
| Trehalose | 8.37 | 10.14 | 9.64 | 7.95 | 9.9815 | 10.5605 | 9.158 |
| 11.8 | 12.25 | 11.58 |
| Xylulose | / | / | / | / |
| 15.6985 |
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| Propanoic acid | 16.04 | 20.68 | 17.27 | 15.65 |
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| n-butyric acid | 18.87 | 24.7 | 20.17 | 18.51 |
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| Isobutyric acid | 17.52 | 23.21 | 18.83 | 17.14 |
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| Butane tetracarboxylic acid | 8.43 | 10.6 | 9.73 | 8 |
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| Sodium acetate | 14.11 | 17.95 | 15.34 | 13.7 |
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| Sodium nitrite | / | / | / | / |
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| Sodium Pyrosulfite | 12.37 | 12.07 | 12.93 | / |
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Test Conditions
Flow Rate: 0.6 mL/min
Detector: RID
Injection Volume: 10 µL
| Column | Mobile Phase | Column Temperature |
| Carbomix H-NP10 | 2.5mM H2SO4 | 80 °C |
| Carbomix H-NP5 | 2.5mM H2SO4 | 55 °C |
| Carbomix Ca-NP10 | Water | 85 °C |
| Carbomix Ca-NP5 | Water | 85 °C |
| Carbomix Pb-NP10 | Water | 75 °C |
| Carbomix Pb-NP5 | Water | 75 °C |
Downloads
Brochures
Sepax Carbomix Carbohydrate & Organic Acid Separation Brochure
Technical Support
Sepax Carbomix Retention Time Table
Template for Communications for the Journal of the American Chemical Society
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