Porvair / Assay Plates

Assay Plates

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Porvair
Assay Plates

Porvair Sciences have a range of clear polystyrene bottom, glass bottom and solid bottom assay plates that are specifically designed for absorbance, fluorescence, luminescence and scintillation applications. All Assay Plates maintaining the standard ANSI/SLAS format.

Microplate readers are designed to read from either the top or the bottom of a microplate. Top reading instruments rely on measuring reflected light above the wells. A good solid bright white plate is best for these absorbance measurements and a solid black plate for fluorescence readings. Bottom reading units illuminate the sample from above and then use detectors placed below the plate to measure the absorption or fluorescence/luminescence emission. This necessitates the use of clear-bottomed plates. The requirement is to transmit the light wavelengths of interest.

Visible wavelength range (900-350nm) measurements require only clear plastic bases, whilst readings between 220nm and 350nm will require a UV-transparent material. This can be Quartz sheet or a special polymer such as Cyclo-Olefin Co-Polymer (COP/COC). Optical glass sheet (borosilicate glass) is used where visible range detection is combined with confocal optics or whole plate imaging which requires a very clear uniformly-flat base.

Clear and opaque polystyrene assay plates are suitable for top reading applications.

Porvair's Krystal™ series includes assay plates for bottom-reading applications (although some of them can be used for top-reading applications as well). Krystal™ assay plates therefore have clear bottoms.

Product Line

Technical Data

The following types of Assay Plates are available:

Clear Solid Polystyrene Assay Plates

96-well plates for routine adsorption-, ELISA-, mixing- and storage applications
Manufactured from high quality crystal polystyrene
Different bottom shapes are available
- Flat for spectrophotometric work
- V-bottom minimizing residual liquid
- U-bottom for cell/particulate collection
Robot compatible
Working volumes from 275μl down to 10μl

Opaque Solid Polystyrene Assay Plates

96-well plates in black, white or black with white wells
384-well plates in black, white and clear
For absorbance, fluorescence, luminescence and scintillation applications
Standard ‘chimney’ wells to overcome optical crosstalk and contamination
The acclaimed composite black/white plate has been shown to be ten times more sensitive than a standard white plate for chemi-luminesence assays.

Krystal™ Black & White Assay Plates

Available as 24-well, 96-well or 384-well version
Opaque walls to prevent well-to-well crosstalk
Clear plate bottom permits direct microscopic viewing
For use with top or bottom reading instruments
96-well Krystal™ 2000 plates are optimised for luminescence and fluorescence assays

Krystal™ polystyrene clear bottom

Standard clear polystyrene bottom plates
Ultrasonically welded for perfect sealing every time
0.4mm thick (384-well)
0.75mm (24- and 96-well)

Krystal™ glass bottom plates

Polystyrene plates with borosilicate glass bottom for whole-plate CCD imaging and laser detection applications
Available as 24-well, 96-well or 384-well version
175μm glass thickness and 335nm UV cut-off
Very low autofluorescence
Biocompatible adhesive
Resistant to alcohol, DMSO and PBS

Krystal™ UV clear bottomed microplates

Ideal for laboratories measuring circular dichroism (CD)
COP- or quartz bottomed, UV-transparent well-plates
Available as 96-well or 384-well version
UV cut-off (COP) at 220nm
Applications e.g. most sensitive UV range fluorescence assays using wholeplate imaging or confocal microscopy

Components for Assay & Method Development

A- and B-plates as well as lids

MZ-Analysentechnik GmbH is official distributor for Porvair Sciences in Germany. For further technical and price information please contact us!

Downloads

Brochures

Sample Preparation
Porvair Assay Plates Brochure 2017

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