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Assay Plates
Porvair Sciences have a range of clear polystyrene bottom, glass bottom and solid bottom assay plates that are specifically designed for absorbance, fluorescence, luminescence and scintillation applications. All Assay Plates maintaining the standard ANSI/SLAS format.
Microplate readers are designed to read from either the top or the bottom of a microplate. Top reading instruments rely on measuring reflected light above the wells. A good solid bright white plate is best for these absorbance measurements and a solid black plate for fluorescence readings. Bottom reading units illuminate the sample from above and then use detectors placed below the plate to measure the absorption or fluorescence/luminescence emission. This necessitates the use of clear-bottomed plates. The requirement is to transmit the light wavelengths of interest.
Visible wavelength range (900-350nm) measurements require only clear plastic bases, whilst readings between 220nm and 350nm will require a UV-transparent material. This can be Quartz sheet or a special polymer such as Cyclo-Olefin Co-Polymer (COP/COC). Optical glass sheet (borosilicate glass) is used where visible range detection is combined with confocal optics or whole plate imaging which requires a very clear uniformly-flat base.
Clear and opaque polystyrene assay plates are suitable for top reading applications.
Porvair's Krystal™ series includes assay plates for bottom-reading applications (although some of them can be used for top-reading applications as well). Krystal™ assay plates therefore have clear bottoms.
Technical Data
The following types of Assay Plates are available:
Clear Solid Polystyrene Assay Plates
- 96-well plates for routine adsorption-, ELISA-, mixing- and storage applications
- Manufactured from high quality crystal polystyrene
- Different bottom shapes are available
- Flat for spectrophotometric work
- V-bottom minimizing residual liquid
- U-bottom for cell/particulate collection
- Robot compatible
- Working volumes from 275μl down to 10μl
Opaque Solid Polystyrene Assay Plates
- 96-well plates in black, white or black with white wells
- 384-well plates in black, white and clear
- For absorbance, fluorescence, luminescence and scintillation applications
- Standard ‘chimney’ wells to overcome optical crosstalk and contamination
- The acclaimed composite black/white plate has been shown to be ten times more sensitive than a standard white plate for chemi-luminesence assays.
Krystal™ Black & White Assay Plates
- Available as 24-well, 96-well or 384-well version
- Opaque walls to prevent well-to-well crosstalk
- Clear plate bottom permits direct microscopic viewing
- For use with top or bottom reading instruments
- 96-well Krystal™ 2000 plates are optimised for luminescence and fluorescence assays
Krystal™ polystyrene clear bottom
- Standard clear polystyrene bottom plates
- Ultrasonically welded for perfect sealing every time
- 0.4mm thick (384-well)
- 0.75mm (24- and 96-well)
Krystal™ glass bottom plates
- Polystyrene plates with borosilicate glass bottom for whole-plate CCD imaging and laser detection applications
- Available as 24-well, 96-well or 384-well version
- 175μm glass thickness and 335nm UV cut-off
- Very low autofluorescence
- Biocompatible adhesive
- Resistant to alcohol, DMSO and PBS
Krystal™ UV clear bottomed microplates
- Ideal for laboratories measuring circular dichroism (CD)
- COP- or quartz bottomed, UV-transparent well-plates
- Available as 96-well or 384-well version
- UV cut-off (COP) at 220nm
- Applications e.g. most sensitive UV range fluorescence assays using wholeplate imaging or confocal microscopy
Components for Assay & Method Development
- A- and B-plates as well as lids
MZ-Analysentechnik GmbH is official distributor for Porvair Sciences in Germany. For further technical and price information please contact us!
Downloads
Brochures
Porvair Assay Plates Brochure 2017
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