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Brownlee
Brownlee is a brand name for HPLC columns manufactured by Perkin Elmer™. The phases are based on high-purity, spherical silica gel and are available in many modifications for a wide variety of applications.
Technical Data
- Brownlee Analytical: HPLC phases for routine analysis based on 110 Å silica
- Brownlee Analytical DB: Fully base-deactivated 140 Å silica for analysis of small molecules as well as small peptides
- Brownlee Aquapore® und Bio: HPLC phases based on 300 Å silica for analysis of biomolecules
- Brownlee Choice™: HPLC phases based on 60 Å silica with high surface area, high carbon loads and high retention
- Brownlee Spheri-5® und Spheri-10®: Small-pore (80 Å, 5 μm), silica based sorbent for separating small molecules. Both monofunctional and polyfunctional C8 and C18 sorbents available.
- Brownlee Validated: High purity HPLC phases with inherent low silanophilic activity. For the analysis of acids, bases and neutral compounds.
- Brownlee SPP: Core-Shell (Fused-Core™) phasen for fast and high resolution separations of various compounds. The particle size is 2.7 µm (Core: 1.7 µm).
1. Brownlee Analytical HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| C18 | C18 | 3, 5 µm | 110 Å | + | 13% | L1 | N/A | 2.5-7.5 | 80 °C |
| C8 | C8 | 5 µm | 110 Å | + | 7% | L7 | N/A | 2.5-7.5 | 80 °C |
| phenyl | phenyl | 5 µm | 110 Å | + | 6% | L11 | N/A | 2.5-7.5 | 80 °C |
| Biphenyl | Biphenyl | 5 µm | 110 Å | + | Proprietary | L11 | N/A | 2.5-7.5 | 80 °C |
| Cyano | Cyano (CN) | 5 µm | 110 Å | + | 4% | L10 | N/A | 2.5-7.5 | 80 °C |
| Amino | Amino (NH2) | 3, 5 µm | 110 Å | - | 2% | L8 | N/A | 2.5-7.5 | 80 °C |
| PAH | C18 | 4 µm | 110 Å | + | Proprietary | N/A | N/A | 2.5-7.5 | 80 °C |
| Silica | - | 5 µm | 110 Å | - | - | L3 | N/A | 2.5-7.5 | 80 °C |
2. Brownlee Analytical DB (U)HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| C18 | C18 | 1.9, 3, 5 µm | 140 Å | + | 11% | L1 | N/A | 2.5-8.0 | 80 °C |
| Aq C18 | C18 | 1.9, 3, 5 µm | 140 Å | Proprietary | 6% | L1 | N/A | 2.5-8.0 | 80 °C |
| Biphenyl | Biphenyl | 1.9, 3, 5 µm | 140 Å | + | 8% | L11 | N/A | 2.5-8.0 | 80 °C |
| Cyano | Cyano (CN) | 1.9, 5 µm | 140 Å | - | 4% | L10 | N/A | 2.5-8.0 | 80 °C |
| PFP Propyl | Pentafluorophenyl | 1.9, 3, 5 µm | 140 Å | + | 6% | L43 | N/A | 2.5-8.0 | 80 °C |
| IBD | Polar group embedded Alkyl | 1.9 µm | 140 Å | + | Proprietary | L68 | N/A | 2.5-8.0 | 80 °C |
| PAH | C18 | 1.9 µm | 140 Å | + | Proprietary | N/A | N/A | 2.5-8.0 | 80 °C |
| Silica | - | 1.9 µm | 140 Å | - | - | L3 | N/A | 2.5-8.0 | 80 °C |
3. Brownlee Aquapore and Bio HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| AP OD-300 (C18) | C18 | 7 µm | 300 Å | + | 10% | L1 | N/A | 2.5-8.0 | 60 °C |
| AP RP-300 (C8) | C8 | 7 µm | 300 Å | + | 5% | L7 | N/A | 2.5-8.0 | 60 °C |
| AP AX-300[1] | Polyetdyleneimine | 7 µm | 300 Å | N/A | N/A | N/A | N/A | 2.0-8.0 | 60 °C |
| AP CX-300[2] | Polyamide coating with carboxymetdyl groups | 7 µm | 300 Å | N/A | N/A | N/A | N/A | 2.0-8.0 | 60 °C |
| Bio C18 | C18 | 5 µm | 300 Å | + | 9% | L1 | N/A | 2.5-8.0 | 80 °C |
| Bio C8 | C8 | 5 µm | 300 Å | + | 5% | L7 | N/A | 2.5-8.0 | 80 °C |
[1] Weak Anion Exchanger [2] Weak Cation Exchanger
4. brownlee Choice™ HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| C18 | C18 | 5 µm | 60 Å | + | 27% | L1 | N/A | 2.5-8.0 | 80 °C |
| Aq C18 | C18 | 5 µm | 60 Å | - | Proprietary | L1 | N/A | 2.5-8.0 | 80 °C |
| Biphenyl | Biphenyl | 5 µm | 60 Å | - | 23% | L11 | N/A | 2.5-8.0 | 80 °C |
| PFP Propyl | Pentafluorophenyl | 3, 5 µm | 60 Å | + | 17% | L43 | N/A | 2.5-8.0 | 80 °C |
| Basix | Phenyl Cyano | 5 µm | 60 Å | + | 12% | L10 | N/A | 2.5-8.0 | 80 °C |
| Organic Acid[1] | N/A | 5 µm | 60 Å | + | Proprietary | N/A | N/A | 2.5-8.0 | 80 °C |
| AK[2] | N/A | 5 µm | 60 Å | - | Proprietary | N/A | N/A | 2.5-8.0 | 80 °C |
[1 ] Special phase for the separation of organic acids
[2] Special phase for the separation of DNPH derivatised aldehydes and ketones
5. Brownlee Spheri-5 and Spheri-10 HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| Spheri-5 C18 | C18 | 5 µm | 80 Å | + | 11% | L1 | N/A | N/A | N/A |
| Spheri-5 C18 ODS (Polyfunctional) | C18 | 5 µm | 80 Å | + | 14% | L1 | N/A | N/A | N/A |
| Spheri-10 C18 | C18 | 10 µm | 80 Å | + | 11% | L1 | N/A | N/A | N/A |
| Spheri-10 C2 | C2 | 10 µm | 80 Å | - | N/A | L16 | N/A | N/A | N/A |
| Spheri-5 Silica | - | 5 µm | 80 Å | N/A | N/A | L3 | N/A | N/A | N/A |
6. Brownlee Validated HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| C18 | C18 | 3, 5 µm | 100 Å | + | 20% | L1 | N/A | 2.5-8.0 | 80 °C |
| Aq C18 | C18 | 3, 5 µm | 100 Å | - | 15% | L1 | N/A | 2.5-8.0 | 80 °C |
| C8 | C8 | 5 µm | 100 Å | + | 12% | L7 | N/A | 2.5-8.0 | 80 °C |
| C4 | C4 | 5 µm | 100 Å | + | 9% | L26 | N/A | 2.5-8.0 | 80 °C |
| C1 | C1 | 5 µm | 100 Å | - | 5% | L13 | N/A | 2.5-8.0 | 80 °C |
| phenyl | phenyl | 3, 5 µm | 100 Å | + | 10% | L11 | N/A | 2.5-8.0 | 80 °C |
| Cyano | Cyano (CN) | 5 µm | 100 Å | + | 8% | L10 | N/A | 2.5-8.0 | 80 °C |
| Amino | Amino (NH2) | 3, 5 µm | 100 Å | - | 2% | L8 | N/A | 2.5-8.0 | 80 °C |
| PFP Propyl | Pentafluorophenyl | 5 µm | 100 Å | + | 11% | L43 | N/A | 2.5-8.0 | 80 °C |
| IBD | Polar group embedded Alkyl | 3, 5 µm | 100 Å | - | 12% | L68 | N/A | 2.5-8.0 | 80 °C |
| Carbamate[1] | N/A | 3, 5 µm | 100 Å | Proprietary | Proprietary | N/A | N/A | 2.5-8.0 | 80 °C |
| Quat[2] | N/A | 5 µm | 100 Å | Proprietary | Proprietary | N/A | N/A | 2.5-8.0 | 80 °C |
[1] Special phase for the analysis of diquat, paraquat and other quaternary amines
[2] Special phase for the analysis of carbamates
7. Brownlee SPP (U)HPLC columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| C18 | C18 | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0-9.0 | 60 °C |
| Peptides ES-C18 | C18 | 2.7 µm | 160 Å | + | N/A | N/A | N/A | 1.0-8.0 | 90 °C |
| C8 | C8 | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0-9.0 | 60 °C |
| Phenylhexyl | Phenylhexyl | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0-9.0 | 60 °C |
| PFP | Pentafluorophenyl | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0-8.0 | 60 °C |
| RP amides | Amide embedded Alkyl | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0-9.0 | 60 °C |
| HILIC | N/A | 2.7 µm | 90 Å | - | N/A | N/A | N/A | 2.0-8.0 | 60 °C |
Downloads
Brochures
Perkin-Elmer Chromatography Consumables and Supplies Catalogue 2017-2018
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