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Nouryon™, formerly known as AkzoNobel Specialty Chemicals, from Sweden is the producer of the famous and well-proven Kromasil® chromatographic silica. The Kromasil® family has been divided into four areas in recent years: Classic, Eternity, Chiral and SFC. The phases are based on high-purity, spherical silica gel with superior mechanical and chemical stability. Kromasil® HPLC columns are available in particle sizes from 1.8 to 16 µm for analytical to preparative applications. Various pore sizes and a large number of modifications cover a wide range of selectivity. Kromasil® silica is also available as bulk material.
The Kromasil® Classic product range includes a variety of high quality HPLC columns with a combination of high pore volume and surface area, together with excellent mechanical and chemical stability. The columns are an effective tool for the separation of a wide variety of substances from small molecules to peptides and proteins.
Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
Kromasil® Classic CN | Cyanopropyl | 5, 10, 16 µm | 60 Å | - | 12% | L10 | 540 m2/g | 2.0‒8.0 | 60 °C |
Kromasil® Classic Diol | Diol | 5, 10 µm | 60 Å | - | 10% | L20 | 540 m2/g | 2.0‒8.0 | 60 °C |
Kromasil® Classic HILIC-D | Diol | 5, 10 µm | 60 Å | - | 10% | L20 | 540 m2/g | 2.0‒8.0 | 60 °C |
Kromasil® Classic SIL | - | 5, 7, 10, | 60 Å | - | - | L3 | 540 m2/g | 2.0‒8.0 | 60 °C |
Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
Kromasil® Classic C18 | C18 | 1.8, 2.5, 3.5, 5, | 100 Å | + | 20% | L1 | 320 m2/g | 2.0‒10.0 | 60 °C |
Kromasil® Classic C8 | C8 | 1.8, 2.5, 3.5, 5, | 100 Å | + | 12% | L7 | 320 m2/g | 2.0‒9.5 | 60 °C |
Kromasil® Classic C4 | C4 | 1.8, 2.5, 3.5, 5, | 100 Å | + | 12% | L26 | 320 m2/g | 2.0‒9.5 | 60 °C |
Kromasil® Classic diC4 | di-Butyl | 10, 13 µm | 100 Å | N/A | N/A | N/A | 320 m²/g | N/A | N/A |
Kromasil® Classic C1 | C1 | 5 µm | 100 Å | + | 8% | L13 | 320 m2/g | 2.0‒8.0 | 60 °C |
Kromasil® Classic Phenyl | Phenylbutyl | 5, 10, 16 µm | 100 Å | + | 4.7% | L11 | 320 m2/g | 2.0‒8.0 | 60 °C |
Kromasil® Classic NH2 | Aminopropyl | 3.5, 5, 7, | 100 Å | - | 14% | L8 | 320 m2/g | 2.0‒9.5 | 60 °C |
Kromasil® Classic SIL | - | 3.5, 5, 7, | 100 Å | - | 1.7% | L3 | 320 m2/g | 2.0‒8.0 | 60 °C |
Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
Kromasil® Classic C18 | C18 | 5, 10, 16 µm | 300 Å | + | 8.7% | L1 | 110 m2/g | 2.0‒9.5 | 60 °C |
Kromasil® Classic C8 | C8 | 5, 10, 16 µm | 300 Å | + | 4.7% | L7 | 110 m2/g | 2.0‒9.5 | 60 °C |
Kromasil® Classic C4 | C4 | 5, 10, 16 µm | 300 Å | + | 2.9% | L26 | 110 m2/g | 2.0‒9.5 | 60 °C |
Kromasil® Classic diC4 | di-Butyl | 10, 16 µm | 300 Å | N/A | N/A | N/A | 110 m2/g | N/A | 60 °C |
Kromasil® Classic SIL | - | 5, 10, 16 µm | 300 Å | - | - | L3 | 110 m2/g | 2.0‒8.0 | 60 °C |
Seperation of 9 peptides with Kromasil 100-5-C8.
1. oxytocin
2. bradykinin
3. methionine enkephalin
4. angiotensin II
5. leucine enkephalin
6. angiotensin I
7. insulin
8. lysozyme
9. melittin
Test Conditions:
Column: Kromasil 100-5-C8 4.6 x 250 mm
Mobile Phase A: acetonitrile/water/TFA (10/90/0.1)
Mobile Phase B: acetonitrile/water/TFA (90/10/0.1)
Gradient:
Time (min) | %B |
0 | 0 |
8 | 25 |
20 | 75 |
Flow rate: 2 mL/min
Temperature: ambient
Detection: UV 254 nm
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