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apHera
ApHera™ are HPLC columns from Merck-Supelco™, with a vinyl alcohol copolymer as base material. These phases were developed to combine the superior advantages of both silica and polystyrene, without the disadvantages of either. They show high stability over a wide pH range and are stable in all common organic solvents. Furthermore the pore size of 300 Å enable the separation of peptides and proteins. The apHera™ NH2 phase is especially suitable for the separation of mono‒ and oligosaccharides.
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Technical Data
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| apHera™ C18 | C18 | 5, 9, 13 µm | 300 Å | N/A | N/A | L67 | N/A | 2.0-12.0 | N/A |
| apHera™ C8 | C8 | 5 µm | 300 Å | N/A | N/A | N/A | N/A | 2.0-12.0 | N/A |
| apHera™ C4 | C4 | 5 µm | 300 Å | N/A | N/A | N/A | N/A | 2.0-12.0 | N/A |
| apHera™ NH2 | Polyamine | 5, 9, 13 µm | 300 Å | N/A | N/A | L82 | N/A | 2.0-12.0 | N/A |
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Applications
Merck Supelco Applications-Notebook Vol1 HPLC, UHPLC, LC-MS, SFC, SPE
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