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Vydac
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The original developer and manufacturer of Vydac-TP‒, Vydac-MS‒, Vydac Everest™‒ and Vydac Denali™ columns was Grace Vydac. Since May 2016, the new manufacturer is the britain specialist Hichrom Ltd.
Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations. The large pores of the 300 Å TP silica give polypeptide molecules complete access to the interior of the silica pores. Other features of Vydac TP Phases are a long column lifetime, a very low phase bleeding and the availability in analytical and preparative dimensions.
Vydac MS columns are a later development from Grace for the reversed-phase HPLC separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins. Vydac MS phases show excellent peak shape with little or no TFA and high protein recovery rates.
Everest™ columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. The high carbon coverage of Everest™ columns increases column life and reduces the amount of TFA required to shield the silica.
Vydac Denali™ (238DE) is a 120Å C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes.
Technical Data
Vydac-TP phases
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| Vydac 201TP C18 | C18 | 5, 10 µm | 300 Å | - | 8% | L1 | 70-90m2/g | N/A | N/A |
| Vydac 202TP C18 | C18 | 5, 10 µm | 300 Å | - | 9% | L1 | 60-90m2/g | N/A | N/A |
| Vydac 218TP C18 | C18 | 3, 5, 10 µm | 300 Å | + | 8% | L1 | 60-110m2/g | N/A | N/A |
| Vydac 238TP C18 | C18 | 5 µm | 300 Å | + | 4% | L1 | 60-110m2/g | N/A | N/A |
| Vydac 208TP C8 | C8 | 5 µm | 300 Å | + | 5% | L7 | 60-110m2/g | N/A | N/A |
| Vydac 214TP C4 | C4 | 5, 10 µm | 300 Å | + | 3% | L26 | 60-110m2/g | N/A | N/A |
| Vydac 214ATP C4 | C4 | 5 µm | N/A | N/A | N/A | L26 | N/A | N/A | N/A |
| Vydac 219TP Di-Phe | diphenyl | 5, 10 µm | 300 Å | + | 4% | L11 | 60-110m2/g | N/A | N/A |
Vydac-MS phases
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| Vydac 218MS C18 | C18 | 5, 10 µm | 300 Å | + | 8% | L1 | 60-110m2/g | N/A | N/A |
| Vydac 238MS C18 | C18 | 5 µm | 300 Å | + | 4% | L1 | 70m2/g | N/A | N/A |
| Vydac 208MS C8 | C8 | 5 µm | 300 Å | + | 5% | L7 | 70m2/g | N/A | N/A |
| Vydac 214MS C4 | C4 | 5, 10 µm | 300 Å | + | 3% | L26 | 70-110m2/g | N/A | N/A |
| Vydac 219MS Diphenyl | Diphenyl | 5 µm | 300 Å | + | 4% | L11 | 70m2/g | N/A | N/A |
Vydac Everest™ phases
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| Vydac 238EV C18 | C18 | 5, 10 µm | 300 Å | + | 6% | L1 | 70-110m2/g | N/A | N/A |
Vydac Denali™ phases
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| Vydac 238DE C18 | C18 | 5, 10 µm | 120 Å | N/A | N/A | L1 | N/A | N/A | N/A |
Downloads
Brochures
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Grace Chromatography Essentials Catalogue
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Grace GC Columns Catalogue
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Grace Alltech Prevail Catalogue
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Grace Alltech Platinum Catalogue
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Grace GraceSmart Catalogue
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Grace Alltech Brava Catalogue
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Grace Alltech Apollo Catalogue
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Grace Alltech Alltima Catalogue
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Grace Alltech Allsphere Catalogue
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Grace Grom Catalogue
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Grace Jones Genesis Catalogue
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Grace Alltech Specialty Columns Catalogue
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Grace Vydac Catalogue
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Grace IC Columns Catalogue
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Grace VisionHT Brochure
The right column for you - we will be happy to support you individually
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