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Avantor-ACE / Avantor ACE HILIC

Avantor ACE HILIC

ACE HILIC-A, ACE HILIC-B and ACE HILIC-N

...these are the latest columns of Advanced Chromatography Technologies Ltd. that have been developed to enable chromatographers to achieve reproducible and successful Hydrophilic Interaction Chromatography, short HILIC, separations using a systematic approach to method development. The columns are available with three unique modifications:

ACE HILIC-A  • Acidic character phase (Proprietary SIL)
ACE HILIC-B  • Basic character phase (Proprietary Aminopropyl)
ACE HILIC-N  • Neutral character phase (Proprietary Polyhydroxy)

Each phase offering alternative selectivity, which allows for a simplified and structured approach to achieving successful HILIC separations. Additionally ACE HILIC Method Development kits are also available. They consist of three ACE HILIC columns, HILIC-A, HILIC-B and HILIC-N, respectively. ACT has created a very useful document for method development support the „ACE Knowledge Note #0021“. A very easy and quick protocol for the method development using the ACE HILIC columns is described in the Knowledge Note.

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ACE 1.7 HILIC-A 30x2.1mm
HILA-17-0302U
ACE 1.7 HILIC-A 20x3.0mm
HILA-17-0203U
ACE 1.7 HILIC-A 20x2.1mm
HILA-17-0202U
ACE 3 HILIC-A 0.5 mm Capillary Guard
HILA3005005GD
ACE 5 HILIC Method Development UHPLC/HPLC Column Kit 75x4.6mm (HILIC-A, HILIC-B AND HILIC-N)
MDKH-5-7546U
ACE 5 HILIC Method Development UHPLC/HPLC Column Kit 75x3.0mm (HILIC-A, HILIC-B AND HILIC-N)
MDKH-5-7503U
ACE 5 HILIC Method Development UHPLC/HPLC Column Kit 75x2.1mm (HILIC-A, HILIC-B AND HILIC-N)
MDKH-5-7502U
ACE 5 HILIC Method Development Microbore HPLC Column Kit 75x1.0mm (HILIC-A, HILIC-B AND HILIC-N) (1/32" Connection)
MDKH-5-7501S
ACE 5 HILIC Method Development Microbore HPLC Column Kit 75x1.0mm (HILIC-A, HILIC-B AND HILIC-N)
MDKH-5-7501
ACE 5 HILIC Method Development Microbore HPLC Column Kit 75x0.5mm (HILIC-A, HILIC-B AND HILIC-N) (1/32" Connection)
MDKH-5-75005S
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Technical Data

The columns are available in nearly all common analytical column lengths. Available column IDs and particle sizes are shown in the following table:

Column type

Inner diameter

Particle size

Analytical

2.1 mm, 3.0 mm, 4.6 mm

1.7 µm, 3 µm, 5 µm

Microbore

0.5 mm, 1.0 mm

3 µm, 5 µm

Applications

β-Blockers using HILIC Mode

Determination of β-blockers using HILIC mode

1. Propanolol

2. Sotalol

3. Acebutolol

4. Salbutamol

5. Atenolol

Caffeine and Related Compounds using HILIC Mode

Determination of Caffeine and Related Compounds using HILIC Mode

1. Caffeine

2. Theophylline

3. Theobromine

4. Xanthine

5. Hypoxanthine

Methylmalonic Acid and Succinic Acid using HILIC Mode

Determination of Methylmalonic Acid and Succinic Acid using HILIC Mode

1. Succinic acid

2. Methylmalonic acid

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