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HALO PCS Phenyl-Hexyl
The HALO PCS Phenyl-Hexyl from AMT (Advanced Material Technologies) is a stationary phase with a positively charged surface (PCS) and a phenyl-hexyl functional group. This positive charge has a particular effect on bases. At low pH values, the bases are positively charged and are repelled by the stationary phase, which leads to better peak shapes, especially at higher loadings. This positive charge also makes the stationary phase ideally suited for mobile phases with a low ionic strength, making this phase ideal for LC-MS.
Applications
- Basic compounds
- Improved peak shape and higher loadability compared to uncharged phenyl-hexyl phases
- Alternative selectivity to HALO PCS C18
Technical Data
| Phase | Modification | Particle size | Pore size | Surface area | Endcapped | USP | Tmax |
| HALO PCS Phenyl-Hexyl | Positively charged surface Phenyl-Hexyl | 2.7 µm | 90 Å | 125 m²/g | + | L11 | 60°C (pH 2) 40°C (pH 7) |
Applications
What characterises the HALO PCS phenyl-hexyl column?
- Outstanding stability under 0.1% formic acid conditions
Column: HALO 90Å PCS Phenyl-Hexyl, 2.7µm, 100x2.1mm
Mobile Phase: 20% Acetonitrile (premixed) with 0.1% Formic acid
Flow rate: 0.6mL/min
Temperature: 60°C
Injection volume: 0.5µL
Detection: 254nm
Instrument: Nexera X2
- Alternative selectivity to HALO PCS C18
Peak Identities
1. Uracil
2. Phenol
3. Oxazepam
4. Flunitrazepam
5. Diazepam
6. 1-Chloro-4-nitrobenzene
7. Naphthalene
- Use of methanol for improved π-π interaction
Peak Identities
1. Uracil
2. Phenol
3. Oxazepam
4. Flunitrazepam
5. Diazepam
6. 1-Chloro-4-nitrobenzene
7. Naphthalene
When do you benefit from HALO PCS Phenyl-Hexyl?
- Higher sample loading of basic compounds with simultaneously lower proportion of the organic phase
Peak Identities
1. Doxepin
2. Nortriptyline
3. Amitryptyline
4. Trimipramine
- Better resolution compared to competitors
Peak Identities
1. Uracil
2. Phenol
3. Oxazepam
4. Flunitrazepam
5. Diazepam
6. 1-Chloro-4-nitrobenzene
7. Naphthalene
Conclusion: The HALO PCS Phenyl-Hexyl offers a better peak shape for basic analytes due to the positively charged surface and can also offer an alternative selectivity to the HALO PCS C18.
Downloads
Brochures
AMT HALO® PCS-Family HPLC Brochure
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