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HALO PCS C18
The HALO PCS C18 series is the latest addition to the HALO product line. This new phase has a positively charged surface (PCS) to provide a better peak shape for basic compounds and is based on AMT's (Advanced Material Technology) fused-core technology. For this reason, the HALO PCS C18 has a higher loading capacity and offers an alternative selectivity compared to other common C18 phases for use with low ionic strength mobile phases. The HALO PCS C18 phase is available in a particle size of 2.7µm in 90 and 160Å and with an inner diameter of 1.5, 2.1, 3.0 and 4.6mm.
Technical Data
| Phase | Modification | Particle size | Pore size | Endcapping | C-content | USP | Surface area | pH range | Tmax |
| HALO PCS C18 | Positively charged surface C18 | 2.7 µm | 90 Å | + | 7.5 | L1 | 135 m²/g | 2.0 - 7.0 | 60°Ca 40°Cb |
| HALO PCS C18 | Positively charged surface C18 | 2.7 µm | 160 Å | + | 5.1 | L1 | 90 m²/g | 2.0 - 7.0 | 60°Ca 40°Cb |
a Low pH; b High pH
Applications
What characterises the HALO® PCS-C18?
- High lot-to-lot reproducibility
HALO 90 Å PCS C18, 2.7µm, 4.6x50mm
Batch-to-batch comparison shows reproducibility and good peak shapes for basic, acidic and neutral compounds
Peak Identities
1. Uracil
2. 2-Chlrobenzoic acid
3. 4-Methoxybenzoic acid
4. Imipramine
5. Dimethyl Phthalate
- Outstanding stability of the HALO PCS C18
HALO 90Å PCS C18, 2.7µm, 2.1x100mm
A screening of antidepressants over 1000 injections (10,000 column volumes) proves the excellent stability of HALO PCS C18.
Test Conditions
Column: HALO 90Å PCS C18, 2.7µm, 2.1x100mm
Part Number: 92812-617
Mobile Phase A: Water, 0.1% Formic acid
Mobile Phase B: Acetonitrile, 0.1% Formic acid
Isocratic: 20% B
Flowrate: 0.6mL/min
Back Pressure: 244bar
Temperature: 60°C
Injection volume: 0.5µL
Sample Solvent: 80/20 Water/Acetonitrile
Detection: PDA, 254nm
Flow Cell: 1µL
Data Rate: 40Hz
Response Time: 0.025sec
LC System: Shimdazu Nexera X2
When do you benefit from the HALO PCS C18 columns?
- Higher sample loading of basic compounds compared to fully porous C18 columns
HALO 90 Å PCS C18, 2.7µm, 2.1x100mm
As shown in this basic drug screening with antidepressants, the HALO® Fused-Core® PCS technology tolerates a higher sample loading with basic substances compared to the competitor's fully porous C18 column. The positively charged surface (PCS) stationary phase is ideal for basic analytes when using low ionic strength mobile phases such as formic acid.
Peak Identities
1. Doxepin
2. Desipramine
3. Imipramine
4. Nortriptyline
5. Amitriptyline
6. Trimipramine
- Better resolution, improved tailing factors and more bottoms compared to competitor SPP C18+ columns
HALO 90 Å PCS C18, 2.7µm, 2.1x100mm
In a direct comparison of SPP columns, HALO® PCS C18 offers better resolution, improved tailing factors and more floors than the leading competitor.
Peak Identities
1. Uracil
2. Imipramine
3. 4-Methoxybenzoic acid
4. 2-Chlorobenzoic acid
- Improved peak shape and resolution for peptides compared to conventional C18 phases
HALO 160 Å PCS C18, 2.7µm, 4.6x100mm
A HALO 160 Å PCS C18 column outperforms a conventional C18 column under formic acid conditions due to its positive charge surface which allows for improved peak shape and resolution for peptides. PCS C18 also allows for higher sample loading on the column for basic analytes and could potentially help pull apart tightly held impurities as seen below.
Peak Identities
1. 5Y Sequence: Ac-RGVVGLYLGK-NH2 (1102 Da)
* Impurity
Conclusion: The HALO® LPH-C18 is suitable for applications with low pH values and also offers fast, efficient and robust separations based on core-shell technology.
As the exclusive distributor of AMT in Germany, you can purchase the new HALO LPH-C18 directly from us. We will be happy to advise and support you in selecting the right column. Contact our support team for advice and a customised quote. We look forward to hearing from you contact us!
Downloads
Brochures
AMT HALO PCS-C18 Brochure
The right column for you - we will be happy to support you individually
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