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HALO OLIGO C18
HALO OLIGO C18 is a new phase from Advanced Materials Technology (AMT). This phase is based on the well-known fused-core technology and has an orragno-silane modified surface for stability at high pH values. Furthermore, this phase is packed in passivated column hardware to prevent adsorption of the analytes on the stainless steel surface. With a pore size of 120Å, HALO OLIGO C18 is suitable for oligonucleotides with up to 60 bases. Due to its high pH and temperature stability, it is ideally suited to the conditions of oligonucleotide analyses.
Technical Data
| Phase | Modification | Particle size | Pore size | Endcapping | C-content | USP | Surface area | pH range | Tmax |
| HALO OLIGO C18 | Organo modified surface C18 | 2.7 µm | 120 Å | + | 5.6 %C | L1 | 75 m²/g | 2.0-9.0 | 90°Ca 85°Cb |
a low pH value; b high pH value
What characterises the HALO OLIGO C18?
High lot-to-lot reproducibility
- Excellent solder-to-solder reproducibility at high pH values and temperatures
Peak Identities
1. 10 mer 2. 15 mer 3. 20 mer 4. 25 mer 5. 30 mer 6. 40 mer 7. 50 mer 8. 60 mer
Column
HALO 120Å OLIGO C18, 2.7µm, 50x2.1mm (P2A62-402)
Stability at high pH value and temperature
- High stability at pH 10 and 60°C
High stability for over 500 injections (20,000 column volumes) with a change in retention of less than 1% at pH 10 and 60°C.
Applications
When will you benefit from the HALO OLIGO C18?
Oligonucleotide separation with good peak shape
- Separation of oligonucleotides up to 60 bases (60 mer) with good peak shape
Peak Identities
1. 10 mer 2. 15 mer 3. 20 mer 4. 25 mer 5. 30 mer 6. 40 mer 7. 50 mer 8. 60 mer * Tris HCl/EDTA
Column
AMT: HALO 120Å OLIGO C18, 2.7µm, 50x2.1mm
Competitor: OLIGO 130Å C18, 1.7µm, 50x2.1mm
Oligonucleotide separation under MS compatible conditions
- Resolution of medium-length synthetic oligonucleotides under MS-compatible conditions
Peak Identities
| Peak Nr. | Peak Identities | ssDNA Sequence | Retention Time | Temperature |
| 1 | T7 Terminator (19 mer) | GCT AGT TAT TGC TCA GCG G | 4.49 min | 60.3 °C |
| 2 | 16s rRNA (20 mer) | AGA GTT TGA TCC TGG CTC AG | 4.68 min | 61.3 °C |
| 3 | T7 Promoter (20 mer) | TAA TAC GAC TCA CTA TAG GG | 4.74 min | 55.2 °C |
| 4 | 16s rRNA (21 mer) | ACG GCT ACC TTG TTA CGA CTT | 4.84 min | 63.5 °C |
| 5 | dT (15 mer) | TTT TTT TTT TTT TTT | 4.99 min | 38.6 °C |
| 6 | dT (16 mer) | TTT TTT TTT TTT TTT T | 5.12 min | 40.9 °C |
Column
HALO 120Å OLIGO C18, 2.7µm, 50x2.1mm
Downloads
Brochures
AMT HALO OLIGO C18 Brochure
Applications
AMT Advantages of HALO OLIGO C18
AMT HALO OLIGO C18 Separation of Oligonucleotide Ladder via LC/MS
AMT HALO OLIGO C18 Oligonucleotide Ladder via UV Detection
AMT HALO OLIGO C18 Oligonucleotide Performance Mix
AMT HALO OLIGO C18 Lot-to-Lot Reproducibility
The right column for you - we will be happy to support you individually
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Application support
Availability and delivery times
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Certificates of analysis, MSDS, data sheets