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Microlute SLE
Microlute™ Supported Liquid Extraction (SLE) is a method of separation based on liquid-liquid extraction, but with one phase immobilised onto an inert support, in this case, diatomaceous earth (DE). The Porvair Sciences Microlute SLE plates show high recovery rates and are designed for the quick and cost-effective clean-up of biological materials prior to LC/MS analysis and work in harmony with our vacuum manifolds and deep-well collection plates. The Microlute SLE Plates are available in standard 96-well format, which allows easy automation during sample preparation.
Product Line
Technical Data
Typical applications:
- Removal of phospholipids, proteins and salts from biological samples, such as serum, plasma and urine
- Dye clean-up in textiles
- Enviromental and food analysis
- Removal of small amounts of water from solvents which are immiscible with water
Protocol:
1. The aqueous sample is loaded into the well.
2. It is allowed to soak into the bed for ~ 5min under the influence of gravity.
3. An organic solvent is used to elute analytes from the media.
4. An organic eluent is used to elute analytes from the media, leaving proteins, phospholipids and salts.
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