• 3% Nachlass bei Online-Bestellung
  • Schnelle Lieferzeiten
  • DIN ISO 9001:2015 zertifiziert
  • Herstellerexpertise
  • Language
  • Mein Warenkorb
  • Account
  • Express-Bestelleingabe

HPLC USP-Methoden und USP-Listing

Adjustments - Anpassungen nach USP Kapitel 621

Adjustments - Anpassungen nach USP Kapitel 621

Anwender aus dem regulierten Umfeld (z. B. der Pharmaindustrie) sind vielfach an sogenannte Monographie-Methoden der europäischen- oder US-Pharmakopöe (Ph. Eur. bzw. USP) gebunden. Die in den Arzneibüchern festgelegten Spezifikationen für die Analytik der zu untersuchenden Stoffe müssen befolgt werden. Diese sind hauptsächlich im Kapitel 621 der USP festgehalten.

Das Kapitel 621 der USP beinhaltet ausdrücklich praxisnahe Empfehlungen, welche Abweichungen von Spezifkationen der Methode in einer Monografie erlaubt, ohne dass eine Revaliderung der Methode erforderlich ist. Das „Adjustment“ (Anpassung) gilt als notwendig für die Erfüllung der Systemeignungstests-(SST)-Kriterien. Die folgenden Tabellen geben einen Überblick über variierbare Parameter mit den jeweils erlaubten Grenzen.

Folgende Parameter dürfen sowohl für isokratische als auch für Gradiententrennungen angepasst werden:

 

ParameterErlaubte Abweichung*
PackungsmaterialBeliebig (solange SST Kriterien erfüllt werden und die Säulenkategorie (LXX) gleich bleibt ("remaining same chemistry")
InjektionsvolumenBeliebig (solange SST-Kriterien erfüllt werden)
Säulentemperatur+/− 10 °C
pH-Wert Mobile Phase+/− 0,2 pH-Einheiten
Salzkonzentration im Puffer / Pufferstärke+/− 10%
Eluentenzusammensetzung

Die Anpassungen gelten für die Unterschusskomponente der Mobilen Phase (≤50%). Der Gehalt dieser Komponenten darf um +/− 30% (relativ) angepasst werden. Die Änderung für jede Komponente darf jedoch 10% absolut (bezogen auf 100% der mobilen Phase) nicht überschreiten.
Folgende Beispiele aus dem Kapitel 621 der USP erläutern dies:

  1. Die Eluentenzusammensetzung beträgt 50:50.
    30% von 50 ist 15% absolut. Dies überschreitet die erlaubte Absolutabweichung von 10%. Eine Anpassung ist somit hier im Bereich von 40:60 bis 60:40 erlaubt.
  2. Die Eluentenzusammensetzung beträgt 2:98.
    30% von 2 ist 0.6% absolut. Die maximal erlaubte Anpassung ist somit hier im Bereich von 1.4:98.6 bis 2.6:97.4.
  3. Die Eluentenzusammensetzung beträgt 60:35:5.
    Für die zweite Komponente: 30% von 35 ist 10.5% absolut. Dies überschreitet die erlaubte Absolutabweichung von 10%. Komponente 2 kann somit lediglich in den Bereich von 25% bis 45% absolut variiert werden.

Für die dritte Komponente: 30% von 5 ist 1.5% absolut. Folgende Anpassungen genügen hier somit den Anforderungen: 50:45:5 bis 70:25:5 oder 58.5:35:6.5 bis 61.5:35:3.5.

 

Folgende Parameter dürfen nur bei isokratischen Trennungen angepasst werden:  

ParameterErlaubte Abweichung*
Flussgeschwindigkeit (F)(1.) +/− 50%
oder
(2.) Eine Flussänderung, die einer vorgenommenen Änderung der Säulenlänge, des Innendurchmessers und der Teilchengröße angepasst ist, siehe weiter unten.
Säulenlänge (L) / Teilchengröße (dp)Erlaubt ist eine Änderung, sodass der neue Quotient L/dp einen Wert zwischen −25% und 50% des Wertes beim ursprünglichen Quotienten entspricht.
Alternativ dazu können auch andere Quotienten (L/dp) verwendet werden, solange die theoretische Bodenzahl N einen Wert zwischen −25% und 50% des ursprünglichen Quotienten einnimmt.
Flussgeschwindigkeit (F) / Teilchengröße (dp)

Wenn die Teilchengröße geändert wird, so muss die Flussgeschwindigkeit angepasst werden, da kleinere Partikel höhere lineare Flussgeschwindigkeiten benötigen, um die gleiche Leistungsfähigkeit zu erreichen (bestimmt durch die theoretische Bodenzahl).
Mithilfe der folgenden Formel kann der neue Fluss bei einer Änderung des Innendurchmessers und der Partikelgröße berechnet werden.

F2 = F1 x [(dc22 x dp1)/(dc12 x dp2)]

F1...Ursprüngliche Flussgeschwindigkeit
F2...Neue Flussgeschwindigkeit
dc1...Säuleninnendurchmesser der urprünglichen Säule
dc2...Säuleninnendurchmesser der neuen Säule
dp1...Urprüngliche Teilchengröße
dp2...Neue Teilchengröße

Beträgt die ursprüngliche Teilchengröße ≥ 3 μm und die neue < 3 μm, so muss der Fluss entsprechend angepasst werden, dass die Bodenzahl nicht mehr als 20% abnimmt.

Säulen-ID

Kann beliebig angepasst werden, unter der Vorraussetzung, dass die lineare Flussgeschwindigkeit konstant bleibt.

Injektionsvolumenbeliebig (solange SST-Kriterien erfüllt werden)

* Modifikationen außerhalb der angegebenen Grenzen gelten als "changes", die eine Revalidierung der eingesetzten Methode erfordern 

 

Nicht geändert werden darf die Wellenlänge des UV/VIS-Detektors. Es muss sichergestellt sein, dass der absolute Fehler bei der Einstellung der Wellenlänge +/− 3 nm nicht überschreitet.

Für genauere Informationen siehe nebenstehende Informationen der USP. ->

USP-Listing

USP-Listing

Die folgende offizielle Liste der HPLC-Packungsmaterialien gemäß USP 621 versteht sich als Auswahlhilfe bei der Wahl einer passenden Säule für die Umsetzung einer USP-Methode. Alle aufgeführten Säulen können direkt bei MZ-Analysentechnik GmbH bestellt werden; sofern existent findet sich zu jeder Methode mindestens ein Packungsmaterial. Die Liste erhebt keinen Anspruch darauf, alle USP-konformen Säulen aufzuführen. Im Sinne möglichst reibungsloser Arbeitsabläufe haben wir den Fokus auf die Auflistung von schnell und zuverlässig lieferbaren Säulen gelegt. Dies garantiert eine möglichst unterbrechungsfreie Arbeit, falls eine Säule ersetzt werden muss.

USP NummerBeschreibungSäulenempfehlung
L1Octadecyl silane chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.PerfectSil 100 ODS-3
PerfectSil Target ODS-3
PerfectSil Target ODS-3 HD
L2Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50 µm in diameterBondapak Prep C18
L3Porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.PerfectSil 120 Sil
PerfectSil 300 Sil
L4Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameterPorasil Prep Silica
SMT Prep Silica
L5Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter- keine erhältlich -
L6Strong cation-exchange packing-sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 µm in diameterPartisil SCX
L7Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.PerfectSil 100 C8-3
PerfectSil 120 C8
PerfectSil 300 C8
L8An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod.PerfectChrom 100 NH2
PerfectSil 120 NH2
L9Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter.Inertsil CX
Partisil SCX
Spherisorb SCX
L10Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.PerfectSil 120 CN
PerfectSil Target CN-3
L11Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.PerfectChrom 100 Phenyl
PerfectSil 120 Phenyl
L12A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50 µm in diameterAccell Plus QMA (SPE)
SMT SAX
L13Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameterPerfectBond C1
PerfectSil 120 C1
L14Silica gel having a chemicallly bonded strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm in diameter.

Inertsil AX
Hypersil SAX
Partisil SAX
Spherisorb SAX

L15Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 µm in diameterPerfectChrom 100 C6
L16Dimethylsilane chemically bonded to porous silica particles, 5 to 10 µm in diameterChromegabond C2
Nucleosil C2
L17Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 µm in diameterICSep COREGEL 87H3
SUGAR SH1011
Carbomix H-NP5
L18Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 µ in diameterChromegabond A/CN
Partisphere PAC
L19Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 5 – 15 µm in diameterCarboSep Coregel-87C
SUGAR SC1011
Carbomix Ca-NP5
L20Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.PerfectChrom 100 Diol
PerfectSil 300 Diol
L21A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 µm in diameterHamilton PRP-1
TSKgel HXL und HHR
PLRP-S
L22A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5 – 15 µm in diameter.ICSep COREGEL 87H3
CarboSep Coregel-87C
SUGAR SC1011
L23An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, 7 - 12 µm in sizeAllsep Anion
TSKgel Q-STAT
L24Polyvinylalcohol chemically bonded to porous silica particles, 5 µm in diameterYMC-Pack PVA-Sil
L25Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) was found suitableTSKgel G2500PWxl
OHpak SB-802.5 HQ
L26Butyl silane chemically bonded to totally porous or superficially porous silica particles, 1.5 to 10 µm in diameterPerfectSil 120 C4
PerfectSil 300 C4
L27Porous silica particles, 30 to 50 µm in diameterPerfectBond Si
L28A multifuncional support, which consists of a high purity, 100 Ǻ, spherical silica substrate that has been bonded with anionic exchanger, amine funcionatily in addition to a conventional reversed phase C8 funcionalityProTec-RP C8
L29Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5 µm in diameter with a pore volume of 80 Ǻ unitsGammaBond ARP-1
L30Ethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameterChromegabond C2
Nucleosil C2
L31A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5-µm macroporous particles having a pore size of 2000 Ǻ units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzeneMCI Gel SCA04
L32A chiral ligand-exchange resin packing-L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 µm in diameterCHIRALPAK WH
MCl Gel CRS10W
L33Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stabilityTSKgel SWxl
Shodex KW400
L34Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, 7 to 9 µm in diameterCARBOSep Coregel-87P
SUGAR SP0810
L35A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150 Ǻ.Zorbax GF-250
L36A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5-µm aminopropyl silicaNucleosil Chiral-3
L37Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a polymethacrylate gelTSKgel G3000PWxl
OHpak SB-803 HQ
L38A methacrylate-based size-exclusion packing for water-soluble samplesTSKgel PWxl
OHpak SB-800HQ
Ultrahydrogel series
L39A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resinTSKgel PWxl
OHpak SB-800HQ
L40Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 3 µm to 20 µm in diameterCHIRALCEL OD-H
Kromasil CelluCoat
L41Immobilized α1-acid glycoprotein on spherical silica particles, 5 µm in diameterCHIRALPAK AGP
L42Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 µm in diameterChromegabond PSC
Hichrom RPB
L43Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameterHALO PFP
Discovery HS F5
InertSustain PFP
L44A multifuncional support, which consists of a high purity, 60 Ǻ, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid funcionality in addition to a convention reversed phase C8 funcionalityChromegabond RP-SCX
Generik C8/SCX
L45Beta cyclodextrin, R,S-hydroxypropyl ether derivative,  bonded to porous silica  particles, 3 to 10 µm in diameterUltron ES-CD
ChiraDex
L46Polystyrene/divinylbenzene substrate agglomerated with quaternary amine funcionalized latex beads, about 9 µm to 11 µm in diameterICSep AN1
L47High capacity anion-exchange microporous substrate, fully functionalized with a trimethylamine group, 8 µm in diameterHamilton PRP-X100
Hamilton RXC-10
Hamilton RXC-30
L48Sulfonated, cross-linked  polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 5 to 15 µm in diameterDionex IonPac AS7
L49A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 to 10 µm in diameterZirChrom PBD
Discovery Zr-PBD
L50Multifunction resin with reverse-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm in diameter, and a surface are of not less than 350 m2 per g. Substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzeneProteomix SAX-POR
L51Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 3 to 10 µm in diameterCHIRALPAK AD-H
Kromasil AmyCoat
L52A strong cation exchange resin made of porous silica with sulfopropyl or sulfoethyl groups, 1 to 10 µm in diameterBioBasic SCX
Supelcosil LC-SCX
TSKgel SP-2SW
L53Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 µEq/columnDionex IonPac CS14
L54A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, 5 – 15 µm in diameter.SUPERDEX 75 10/300 GL
L55A strong cation exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5 µm in diameterIC-Pak C M/D
L56Propyl silane chemically bonded to totally or superficially porous silica particles, 3 to 10 µm in diameterZorbax StableBond C3
L57A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with a pore size of 120 Ǻ.Ultron ES-OVM
L58Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 µm  diameterCARBOSep Coregel 87N
SUGAR KS-802
Carbomix Na-NP5
L59Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is spherical 1.5 - 10 µm, silica or hybrid packing with a hydrophilic coating.TSKgel SWxl
PROTEIN KW-800
L60Spherical, porous silica gel, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and endcappedHalo RP-Amide
Discovery RP-Amide C16
L61A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of  13 µm microporous particles having a pore size less than 10 Ǻ units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quaternary ammonium ions (6%).Dionex IonPac AS-11
L62C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter.SMT C30 Acclaim C30
L63Glycopeptide teicoplanin linked through multiple covalent bonds to a 100 Ǻ units spherical silicaAstec Chirobiotic T
L64Strongly basic anion exchange resin consisting of 8% crosslinked styrene divinylbenzene copolymer with a quaternary ammonium group in the chloride form, 45 to 180 µm in diameterAG1-X8
L65Strongly acidic cation exchange resin consisting of 2% sulfonated crosslinked styrene divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 63 to 250 µm in diameterAG50W-X2
L66A crown ether coated on a 5 µm particle size silica gel substrate. The active site is (S) -18-crown-6-etherCrownpak CR (+)
L67Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10 µm in diameter.apHera C18
Asahipak ODP-40
L68Spherical, porous silica, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcappedTSKgel Amide-8
SUPLEX pKb-100
L69Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 130 nm latex beads, about 6.5 µm in diameter.Dionex CarboPac PA20
L70Cellulose tris(phenyl carbamate) coated on 5 µm silicaChiralcel OC-H
L71A rigid, spherical polymetacrylate, 4 to 6 µm in diameterRSpak DE-413
L72(S)-phenylglycine and 3,5-dinitroanaline urea linkage covalently bonded to silicaSumichiral OA-3300 S
L73A rigid, spherical polydivinylbenzene particle, 5 to 10 µ in diameterMCI GEL CDR10
L74A strong anion-exchange resin consisting of a highly cross-linked core of 7-µm macroporous particles having a 100 Angstroms average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quaternary ammonium ions.Dionex IonPac AS14A
L75A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7 µm in diameter, with a pore size of 300 AngstromsResolvosil BSA
L76Silica based weak cation-exchange material, 5 µm in diameter. Substrate is surface polymerized polybutadiene-maleic acid to provide carboxylic acid functionalities. Capacity not less than 29 µEq/columnIC YK-421
L77Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 6 to 9 µm diameter. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 500 µEq/column (4 mm x 25 cm)Dionex IonPac CS17
L78A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, or tertiary amino groups) functional groups chemically bonded to porous or non-porous or ceramic micro-particles, 1.0 to 50 µm in diameter or a monolithic rodAcclaim Mixed-Mode WAX-1
Primesep B2
L79A chiral-recognition protein, human serum albumin (HSA), chemically bondend to silica particles, about 5 µm in diameter.CHIRALPAK HS
L80Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 - 20 µ in diameterChiralcel OJ-H
L81A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of  9 µm porous particles having a pore size of 2000 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 70 nm diameter microbeads (6% crosslinked) bonded with alkanol quaternary ammonium ionsDionex IonPac AS11-HC
L82Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 4 - 5 µm in diameterAsahipak NH2P-40
L83A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 10.5 µm microporous particles having a pore size of 10 Angstroms and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene.Dionex IonPac AS17-C
L84Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5 µm diameter. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 8400 µEq.column (5 mm x 25 cm).Dionex IonPac CS16
L85A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 1.0 to 50 µm in diameterAcclaim Mixed-Mode WCX-1
Primesep 100
L86Fused core particle with a highly polar ligand possessing multiple hydroxyl groups tethered to the silica gel outer layerAscentis Express OH5
L87Dodecyl silane chemically bonded to porous or superficially silica particles, 1.5 to 10 µm in diameterAccucore RP-MS
L88Glycopeptide vancomycin linked through multiple covalent bonds to 100 Ansgtroms spherical silica.Astec Chirobiotic-V
L89Packing having the capacity to separate compounds with a molecular weight range from 100 – 3000 (as determined by polyethylene oxide), applied to neutral and anionic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylate ether (surface contains some residual cationic functional groups).TSKgel SuperOligoPW
L90Amylose tris-[(S)-alpha-methylbenzylcarbamate] coated on porous, spherical silica particles, 3 to 10 µm in diameterCHIRALPAK AS-H
L91Strong anion-exchange resin consisting of monodisperse porous polystyrene/divinyl benzene beads coupled with quaternary amine. Bead size is 3 - 10 µm.Metrosep A Supp 1
Mono Q 5/50 GL
L92A strong anion-exchange resin consisting of a highly cross-linked core of 5 – 9 µm macroporous particles having a 100 Angstroms average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion exchange layer grafted to the surface, which is functionalized with alkanol quaternary ammonium ions.Dionex IonPac AS15
L93Cellulose tris(3,5-dimethylphenylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particles.CHIRALCEL OD-RH
L94A strong anion-exchange resin consisting of a highly crosslinked 15 µm microporous particles functionalized with very low crosslinked latex (0.5%) to provide alkanol quaternary ammonium ion exchange sites.Dionex IonPac AS4A
L95Highly polar alkyl ligand comprising five hydroxyl groups that are chemically bonded to totally porous or superficially porous silica or a monolithic silica rod.Halo Penta-HILIC
L96Alkyl chain, reversed-phase bonded totatlly or superficially porous silica designed to retain hydrophilic and other oplar compounds when using highly aqueous mobile phases, including 100% aqueous, 1.5 µm to 10 µm in diameter.Zorbax SB-Aq
L97Weak cation-exchange resin consisting of a highly cross-linked core of 5.5 µm porous particles having a pore size of 2000 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 2400 µEq/column (4 mm x 25 cm).Dionex IonPac CS19
L98Weak cation-exchange resin consisting of a highly cross-linked core of 8 µm microporous particles having an average pore size of 10 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. Substrate is surface grafted with carboxylic acid functionalized groups. Capacity not less than 46 µEq/column (4 mm x 5 cm).Dionex IonPac CG19
L99Amylose tris-(3,5-dumethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameterCHIRALPAK IA
L100A 55% crosslinked, microporous, hydrophobic resin core (9 µm microporous particles having a pore size of 10 Angstroms units) that consists of a bilayer of anion and cation exchange latex. The first layer is fully sulfonated (140 nm) and the second layer is fully aminated (76 nm)Dionex IonPac CS5A
L101Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.Cogent UDC-Cholesterol
L102(Naproxen, (S,S)Whelk-O 1) 1-(3,5-dinitrobenzamido)-1,2,3,4-tetrahydrophenanthrene covalently bonded to porous spherical silica particles, 5 to 10 µm in diameter(S,S) Whelk-O 1
L103A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 µm porous particles having a pore size of 2000 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene electrostatically bonded with hyperbranched alkanol quaternary ammonium ionsDionex IonPac AS19
L104Triazole groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameterCosmosil HILIC
L105A strong anion-exchange resin consisting of a highly cross-linked 9 µm supermacroporous (2000 Angstroms) particles functionalized with very low cross-linked latex (0.2%) to provide alkyl quaternary ammonium ion sitesDionex IonPac AS12A
L106Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5-8 µm diameter, macroporous particles having an average pore size of 100 Ǻ units. Substrate is surface grafted with carboxylic acid and phosphonic acid functional groups. Capacity not less than 2800 µEq/column (4 mm x 25 cm).Dionex IonPac CS12A
L107Cellulose tris(4-methylbenzoate)-coated porous spherical particles, 3 to 5 µm in diameter, for use with reversed phase mobile phases.CHIRALCEL OJ-RH
L108A chiral-recognition protein, cellobiohydrolase (CBH), chemically bonded to silica particles, about 5 µm in diameterChiralpak CBH
L109Spherical particles of porous graphitic carbon, 3 to 30 µm in diameterHypercarb
L110A strong anion-exchange resin consisting of a highly cross-linked 13 µm microporous (tess than 10 Angstroms) particles coated with very low cross-linked latex (0.5%) to provide alkanol quaternary ammonium ion exchange sites.Dionex IonPac AG12A
L111Polyamine chemically bonded to porous spherical silica particles, 5 µm in diameterYMC-Pack Polyamine II
L112A hydroxide-selective , strong anion-exchange resin consisting of a highly cross-linked core of 8.5 µm porous particles having a pore size of 2000 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (5% crosslinked) bonded with alkanol quaternary ammonium ionsDionex IonPac AG10
L113A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 7.5 µm porous particles having a pore size of 2000 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (5%) crosslinked bonded with alkanol quaternary ammonium ions.Dionex IonPac AS18
L114Sulfobetaine graft-polymerized to totally or superficially porous silica, 1.5 to 10 µm in diameter, or a monolithic rod. Packing having densely bonded zwitterionic groups with 1:1 charge balanceSeQuant ZIC-HILIC
L115Ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with quaternary amine functionalized 275 nm latex microbeads (6% cross-linked), about 8.5 µm in diameterDionex CarboPac PA100
L116Sulfonated ethylvinylbenzene/divinylbenzene substrate agglomerated with hydrophilic quaternary amine functionalized glycidyl-derivative methacrylate microbeads, approximately 2 to 50 µm in diameterDionex DNAPac PA100
L117A crown ether coated on a 5 µm particle size silica gel substrate. The active site is (R)-18-crown-6-etherCROWNPAK CR(-)
L118Aqueous polymerized C18 groups on silica particles, 1.2 to 5 µm in diameterMZ-PAH
L119Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameterCHIRALPAK IC
L120A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of  13 µm microporous particles having a pore size of <10 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 65 nm diameter microbeads (8% crosslinked) bonded with alkanol quaternary ammonium ions.  Capacity not less than 10 µEq/column (4 mm x 5 cm).Dionex IonPac AG18
L121A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 11 µm porous particles having a pore size of less than 10 Angstroms units and consisting og ethylvinylbenzene cross-linked with 55% divinylbenzene electrostatically bonded with hyperbranched alkanol quartenary ammonium ionsDionex IonPac AG19
L122Sulfobetaine graft-polymerized to totally or superficially porous hydrophilic polymer particles, 1.0 to 10 µm in diameter, or a monolithic rod. Packing having densely bonded zwitterionic grooups with 1:1 charge balance.SeQuant ZIC-pHILIC

Sonderangebote

Sonderangebote

Beim Kauf einer HALO PFAS oder PAH Säule erhalten Sie vom 15. September 2020 bis 31. März 2021 40% Einführungsrabatt.

Mehr lesen ...

Trennungsstrategien für Enantiomere von Daicel™ kostenlos erhältlich.

Mehr lesen ...

Die Buchserie HPLC-Tipps von Dr. Stavros Kromidas ist eine wertvolle Hilfe im Alltag der HPLC-Anwender.

Mehr lesen ...

Neuigkeiten

Neuigkeiten

Exzellente Selektivität für fluorierte Verbindungen von AMT.

Mehr lesen ...

Einfache und präzise Trennung von aromatischen und aliphatischen Kohlenwasserstoffen

Mehr lesen ...

Neu entwickelte Ghost-Guard Säule von Welch speziell für die effektive Entfernung unpolarer Verunreinigungen.

Mehr lesen ...

Neue stationäre Phase für die Analyse von Glykanen. Bis 31. Oktober 2020 sind die neuen Avantor ACE Excel Glykan Säulen mit einem Einführungsrabatt...

Mehr lesen ...

Neue stationäre Phase für die Analyse von Oligonucleotiden, bis 31. Oktober 2020 sind die neuen Avantor ACE Excel Oligo Säulen mit einem...

Mehr lesen ...

HALO PAH - Exzellente Selektivität für PAH Compounds

Mehr lesen ...

Die hochstabile C1 Phase ist vielseitig verwendbar und zeigt exzellente Selektivitäten für unterschiedliche Verbindungsklassen

Mehr lesen ...


  • manufacturer_lable_advanced_chromatography_technologies.jpg
  • Bonna-Agela_W200px.jpg
  • manufacturer_lable_advanced_materials_technology.jpg
  • kromasil_kl.jpg
  • Bio-Rad_kl.jpg
  • CERI_kl.jpg
  • CHROMANIK_kl.jpg
  • CONCISE-SEP_kl.jpg
  • CHIRAL_kl.jpg
  • dikma_kl.jpg
  • ES_kl.jpg
  • GLScieces_kl.jpg
  • gracevydac_kl.jpg
  • HiChrom_kl.jpg
  • IDEX_kl.jpg
  • Imtakt_kl.jpg
  • K-Prime_kl.jpg
  • MACHEREY-NAGEL_kl.jpg
  • MerkMillipore_kl.jpg
  • Micro-Solv_kl.jpg
  • mitsubishi_kl.jpg
  • PERKIN-ELMER_kl.jpg
  • princeton_kl.jpg
  • RESTEK_kl.jpg
  • sepax_kl.jpg
  • Trajan_kl.jpg
  • Shimadzu_kl.jpg
  • Shinwa_kl.jpg
  • shiseido_osaka-soda_kl.jpg
  • SHODEX_kl.jpg
  • supelco_kl.jpg
  • Thermo_kl.jpg
  • TOSOH_kl.jpg
  • waters_kl.jpg
  • welch_kl.jpg
  • YMC_kl.jpg