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Avantor ACE HILIC
ACE HILIC-A, ACE HILIC-B and ACE HILIC-N
...these are the latest columns of Advanced Chromatography Technologies Ltd. that have been developed to enable chromatographers to achieve reproducible and successful Hydrophilic Interaction Chromatography, short HILIC, separations using a systematic approach to method development. The columns are available with three unique modifications:
ACE HILIC-A • Acidic character phase (Proprietary SIL)
ACE HILIC-B • Basic character phase (Proprietary Aminopropyl)
ACE HILIC-N • Neutral character phase (Proprietary Polyhydroxy)
Each phase offering alternative selectivity, which allows for a simplified and structured approach to achieving successful HILIC separations. Additionally ACE HILIC Method Development kits are also available. They consist of three ACE HILIC columns, HILIC-A, HILIC-B and HILIC-N, respectively. ACT has created a very useful document for method development support the „ACE Knowledge Note #0021“. A very easy and quick protocol for the method development using the ACE HILIC columns is described in the Knowledge Note.
Technical Data
The columns are available in nearly all common analytical column lengths. Available column IDs and particle sizes are shown in the following table:
| Column type | Inner diameter | Particle size |
| Analytical | 2.1 mm, 3.0 mm, 4.6 mm | 1.7 µm, 3 µm, 5 µm |
| Microbore | 0.5 mm, 1.0 mm | 3 µm, 5 µm |
Applications
β-Blockers using HILIC Mode
Determination of β-blockers using HILIC mode
1. Propanolol
2. Sotalol
3. Acebutolol
4. Salbutamol
5. Atenolol
Caffeine and Related Compounds using HILIC Mode
Determination of Caffeine and Related Compounds using HILIC Mode
1. Caffeine
2. Theophylline
3. Theobromine
4. Xanthine
5. Hypoxanthine
Methylmalonic Acid and Succinic Acid using HILIC Mode
Determination of Methylmalonic Acid and Succinic Acid using HILIC Mode
1. Succinic acid
2. Methylmalonic acid
Downloads
Brochures
Avantor ACE HILIC UHPLC and HPLC Columns
Applications
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ACE b-blockers HILIC
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ACE caffeine and related compounds HILIC
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ACE comparing selectivity in hilic and rplc modes
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ACE creatine and metabolite creatinine HILIC
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ACE deoxynucleosides HILIC UV-MS
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ACE enhanced selectivity differences on hilic range
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ACE Methylmalonic acid and succinic acid HILIC
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Avantor ACE HILIC Method Development in Chromatography Today
Technical Support
Avantor ACE Method Development Protocol - Step-by-Step HILIC
Avantor ACE Knowledge Note 21 - Step-by-Step Protocol for HILIC Method Development
Avantor ACE Knowledge Note 25 - Column Equilibration in HILIC Mode
Avantor ACE Knowledge Note 24 - Importance of sample diluent in HILIC
The right column for you - we will be happy to support you individually
Competent consultants are always at your side. Write a message to our consultants, we will get back to you and give you individual support.
You will find:
Application support
Availability and delivery times
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Certificates of analysis, MSDS, data sheets